Carnoy液和三氯醋酸对兔上颌窦作用的实验研究

Study of effects of Carnoy's solution and trichloroacetic acid on maxillary sinus of rabbit.

目的 :本实验采用Carnoy液和三氯醋酸 (TCA)涂布兔上颌窦粘膜及窦腔骨壁 ,研究两种药液对这些组织的损伤特性以及对粘膜再生修复和骨改建过程的影响。方法 :实验动物分成 4组 ,分别用Carnoy液、2 0 %和 35 %TCA、生理盐水涂布于双侧上颌窦窦腔 ,术后采用三种荧光素标记新骨 ,制作常规组织切片及非脱钙切片 ,并测量矿化沉积率。结果 :Carnoy液、2 0 %和 35 %TCA都可引起粘膜上皮坏死 ,术后 4周上皮可重新形成。药液作用于刮除粘骨膜的骨创面后 8周未见上皮再生。骨组织计量学测量结果表明新骨沉积过程由于烧灼作用明显抑制。结论 :两种药液具有较强的烧灼作用 ,可致上皮结构破坏 ,抑制上皮再生和骨改建过程。

Objective: The purpose of the study is to examine the effect of Carnoy's solution and trichloroacetic acid(TCA) on maxillary sinus mucosal regeneration and bone remodeling. Methods: Sixteen rabbits were used and divided into four groups: Carnoy's solution, 20%TCA, 35%TCA and control. The rabbit maxillary sinuses were exposed on both sides, and sinus mucoperiosteum was removed unilaterally. The sinuses were spread with the agents about 4 minutes on both sides. Three kinds of fluorochrome were administered respectively at 1,14,28 and 42 day to label the new bone. Sections were made and stained with hematoxylineosin(HE). Mineral appositions rates(MAR) were measured for the specimens of 4,8 weeks by fluorescent microscopy. Results: On the preserved mucosa side, all three kinds of agents caused epithelial necrosis. At 4 weeks mucosa was reepithelialized. The denuded sinuses were not reepithelialized at 8 week postoperatively.The MAR of denuded area treated by Carnoy's solution agent was 2.1μm/day from 2 to 4 weeks and 0.9μm /day from 4 to 6 weeks. Conclusion: Carnoy's solution and TCA are two kinds of cauterizing agents which can cause necrosis of mucosa, inhibit regeneration of mucosa following surgical removal and bone remodeling. [