山西地区61例乙型肝炎病毒耐药变异与基因型检测研究分析

Research on resistance mutations and genotype in 61 cases of hepatitis B virus of Shanxi Province

目的通过对山西省乙型肝炎病毒（hepatitis B virus，HBV）P区基因测序的研究，探讨HBV基因变异位点及基因分型的分布规律。方法采集61例HBV患者血清标本，采用双脱氧末端终止法对待检标本P区进行基因序列检测，用Chromas 2．23软件对测序结果进行分析，通过在NCBI网站进行序列比对，并进行基因分型。结果53例受检患者均检测出P区的碱基以及氨基酸序列图，其中，有28例（52．8％）发生突变，突变位点分别有rt204、rt180、rt236、rt173、rt181、n214、rt250、rt213、rt184、rt237；各突变的位点中，以rt204和rt180位点的突变比例最高，分别占67．9％和53．5％。2例为B基因型，阳性率为3．8％，其余51例均为C基因型，阳性率为96.2％。结论山西省地区HBV分布以C基因型多见；发生变异的HBV病毒株中，10个变异位点均有涉及，但是仍以rt204位点突变为主，伴随其他位点突变，提示rt204位点可能存在顺式作用元件，在HBVDNA基因转录、翻译中起到调控作用。

Objective To discuss genovariation site and genotyping distribution of hepatitis B virus （HBV） in Shanxi Province by dectecting P area gene sequencing of the patients infected with HBV. Methods 61 HBV infected serum samples were collected. The genotyping of P area was detected by the dideoxymediated chain termination method. Sequencing resuhs were analyzed hy Chrnmas 2.23 software, and genotyping were done according NCBI. Results 53 patients all detected the bases and amino acid sequence of the P area. Among them, 28 cases （52.8%） given mutations, there were rt204, rt180, rt236, rt173, rt181, rt214, rt250, rt213, rt184 and rt237 mutations; The rt204 mutation accounts for 67.9% and rtl80 mutation accounts for 53.5%, which were the two toppest mutation rate. 2 cases of B genotype, positive rate was 3.8%, and 51 cases for C genotype, positive rate was 96.2%. Conclusion In Shanxi Province the C genotype is most; 10 sites are all involved, hut still with rt204 site mutation priority, along with other site mutations, which suggesting rt204 site may have the cis-acting element and make effect in gene transcription and translation of HBV DNA.