磷酸二酯酶4D基因单核苷酸多态性与颈动脉斑块易损性的关系

Correlation between the single nucleotide polymorphism of phosphodiesterase 4D gene and instability of carotid plaque

目的 探讨磷酸二酯酶4D（PDE4D）基因SNP83的单核苷酸多态性与颈动脉斑块易损性的关系.方法 收集浙江省台州医院神经内科的脑梗死患者347例,根据颈动脉超声检查结果分为易损斑块组（106例）和稳定斑块组（241例）.采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析PDE4D基因SNP83的单核苷酸多态性.结果 易损斑块组CC＋ CT基因型频率为46.2％,稳定斑块组为33.2％,两组比较差异有统计学意义（P=0.02,OR=1.730,95％CI：1.085～2.759）；易损斑块组C等位基因频率为25.0％,稳定斑块组则为18.5％,两者比较差异有统计学意义（P =0.049,OR=1.472,95％CI：1.000～2.167）.结论 PDE4D基因SNP83单核苷酸多态性可能与浙江地区汉族人群颈动脉斑块的易损性相关,C等位基因可能是颈动脉斑块易损的遗传标志之一.

Objective To analyze the correlation between the single nucleotide polymorphism of SNP83 in phosphodiesterase 4D（PDF4D） gene and instability of carotid plaque.Methods According to the results of carotid B-mode ultrasonography,a total of 347 acute infarction patients from the Department of Neurology of Taizhou Hospital were divided into carotid vulnerable plaque group（106 cases） and carotid stable plaque group （241 cases）.Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was performed on the SNP83 in the PDE4D gene.Results The frequency of the CC ＋ CT genotypes was significantly different between the two groups（46.2％ vs.33.2％,P =0.02,OR =1.730,95％ CI：1.085-2.759）,and the frequencies of the C allele were 25.0％ and 18.5％ in the carotid vulnerable plaque group and the carotid stable plaque group which were also significantly different within the two groups （P =0.049,OR =1.472,95 ％ CI：1.000-2.167）.Conclusion We suggested that the single nucleotide polymorphism of SNP83 in PDE4D gene are associated with carotid vulnerable plaque in Zhejiang Han population,and the C allele may be a predictor for the susceptibility of carotid vulnerable plaque.