HPLC法同时测定2种返魂草药材中金丝桃苷和绿原酸的含量

Simultaneous determination of hyperoside and chlorogenic acid in Fanhuncao by HPLC

目的采用HPLC法同时测定麻叶返魂草和单麻叶返魂草药材中金丝桃苷和绿原酸的质量分数。方法采用Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%(体积分数)三氟乙酸溶液为流动相梯度洗脱,流速为1.0 mL·min^(-1),柱温为30℃,检测波长为280 nm。结果 2种成分均达到基线分离,金丝桃苷和绿原酸的质量浓度分别在1.03～41.02μg·mL^(-1)、2.70～43.20μg·mL^(-1)范围内与峰面积呈良好的线性关系;平均加样回收率分别为97.2%、99.2%,RSD值分别为3.9%、3.9%。麻叶返魂草中这2种成分的质量分数均高于单麻叶返魂草。结论该方法准确、简便、分离度好,为返魂草药材的质量评价提供了依据。

Objective To develop a HPLC method for simultaneous determination of hyperoside and ehlorogenic acid in Senecio cannabifolius and S. cannabifolius vat. integrilifolius. Methods HPLC analysis was carried out on Agilent Zorbax SB-C18 eolunm（4.6 mmx 250 mm,5 μm） with acetonitrile -0.1% trifluoroacetie acid in gradient elution. The flow rate was 1.0 mL - rain L. The column temperature was set at 30 ℃, and the detection wavelength was set at 280 nm. Results Hyperoside was linear in the range from 1.03 to 41.02 μg · mL^-1 （r = 0.999 9） and the average recovery was 97.2% （RSD = 3.9%）. Chlorogenie acid was linear in the range from 2.70 to 43.20μg · mL^-1 （ r = 0. 999 3 ） and the average recovery was 99.2% （ RSD = 3.9% ）. The contents of both them in S. cannabifolius were higher than in S. cannabifolius var. integrilifolius. Conclusion The method is accurate, simple and feasible. It may provide scientific basis for the quality evaluation for Fanhuncao.