羊藿巴戟口服液对去势大鼠雌二醇的影响及机制

Effect of Yanghuobaji oral liquid on estradiol level in ovariectomized rats

目的观察羊藿巴戟口服液对去势大鼠血清雌二醇水平的影响,初步探讨其补肾滋阴作用。方法 48只雌性SD大鼠进行腹腔假手术或切除双侧卵巢,随机分为假手术组、模型对照组、己烯雌酚阳性对照组(2.25×10^(-5)g·kg^(-1))、羊藿巴戟口服液低剂量组(1.0 g·kg^(-1))、中剂量组(3.0 g·kg^(-1))和高剂量组(6.0 g·kg^(-1)),连续给药15 d后,采用HPLC法检测血清雌二醇水平,并解剖称取肾上腺和子宫质量,计算脏器系数。结果与模型对照组比较,羊藿巴戟口服液呈剂量依赖性地增加去势大鼠肾上腺和子宫的脏器系数(P<0.05,P<0.01);羊藿巴戟口服液高剂量组大鼠血清雌二醇水平升高(P<0.05)。结论羊藿巴戟口服液具有一定的补肾滋阴作用。

Objective To investigate the effect of Yanghuobaji oral liquid （YHBJ） on estradiol level and kidney- and-yin replenishing in ovariectomized rats. Methods 48 female SD rats were sham-operated or ovariectomized, and randomly divided into 6 groups including the sham-operated group, the model control group, the diethylstilbestrol group （2.25 × 10 -5 g ·kg^-1） and three YHBJ groups with low （ 1.0 g ·kg^-1） ,middle （3.0 g·kg^-1） and high （6.0 g ·kg^-1） doses. After treatment for 15 days,the level of serum estradiol was detected by high performance liquid chromatography （HPLC）. The organ index of adrenal and uterus were also detected. Results Compared with model control group, YHBJ treatment increased the organ index of adrenal and uterus in ovariectomized rats in a dose-dependent manner （P 〈 0. 05 ,P 〈 0.01 ）. The serum estradiol level in the high-dose YHBJ group was significantly higher than those of the model control group （ P 〈 0. 05 ）. Conclusion YHBJ may contribute to the effect of kidney-and-yin replenishing.