湿片镜检法与革兰染色镜检法在诊断细菌性阴道病中的比较

Comparison of Film Microscopy and Gram Staining Microscopic Inspection in the Diagnose Bacterial Vaginosi

目的 比较湿片镜检法与革兰染色镜检法在诊断细菌性阴道病（bacterial vaginosis,BV）的应用.方法 收集2011年8月～11月经BV速蓝试剂检验结果为阳性的阴道分泌物标本406例进行湿片直接镜检线索细胞;随机选取406例中的210例标本进行革兰染色后镜检线索细胞.两种方法的结果判断以镜下线索细胞≥20%为标准,凡符合此标准的认为与BV速蓝试剂阳性结果符合.结果 406例湿片镜检标本中243例符合判断标准.210例革兰染色镜检标本中127例符合判断标准;湿片镜检法与革兰染色镜检法的符合率分别为59.85%,60.48%,统计结果显示二者差异无统计学意义（χ2=0.22,V=1,P=0.881）.结论 湿片镜检法与革兰染色镜检法在检测线索细胞、辅助诊断细菌性阴道病方面无明显差异,湿片直接镜检是较为理想的方法.

Objective To compare film microscopy and gram staining microscopic inspection in the diagnose bacterial vaginosis （BV）. Methods Collected 406 cases of BV fast blue reagent to detect positive outpatient gynecologic patients in the vaginal secretion. The 406 cases were tested simultaneously microscopic clue cells on wet microscope. Among 406 cases, 210 cases of specimens were selectedrandomly to examine microscopic clue cells after Gram stain. The examine microscopic clue cells was ≥20% that would be considered as consistant with the judgment standardize. Those samles came up to positive coincidence of BV fast blue reagent. Results 243 samples consisted with the jugment standard in the 406 film microscopy samples; 127 samples consisted with the jugment standard in the 210 Gram staining microscopic inspection samples. The comparison be- tween wet film microscopy and Gram staining microscopic inspection with BV fast blue reagent method,which two consis- tence rate were respectively 59.85 %and 60.48%, the statistical results showed that X2= 0. 22, v= 1, P= 0. 881 had no sig- nificant difference. Conclusion The experiment find that there was not significant difference between wet film and Gram staining microscopic examination of clue cell method. The wet film microscopic examination of clue cell maybe a better meth- od to diagnose bacterial vaginosi.