不同破碎方法提取嗜酸乳杆菌细菌素的研究

Study on bacteriocin extracted by different cell disruption methods

采用微波法、珠磨法、超声波法、反复冻融法、石英砂研磨法分别对嗜酸乳杆菌进行细胞破碎分离细菌素,通过研究嗜酸乳杆菌产细菌素抑菌活性来确定最佳细胞破碎物理方法.结果表明,超声波破碎法抑菌圈直径最大,为20.94 mm,细胞破碎效果最好.

Bacteriocin was seperated and got after the cell of Lactobacillus acidophilus was disrupted by the microwave test, bead mill test, ultrasonication, freezing and thawing test, and quartz sand grinding test. The better physical method of the cell disruption was studied by the bacteriostatic activity of bacterioein. The result showed that the diameter of inhibition zone by uhrasonication was the largest, which was 20.94 mm, so the effect of the cell dis- ruption was best.