摘要
目的建立强肝益肾颗粒中黄芪甲苷的HPLC测定方法。方法采用C18柱,温度40℃,甲醇-水（75：25）为流动相;流速1mL/min;蒸发光散射检测器检测,漂移管：108℃;气体流速：2.0L/min;外标两点法定量。结果黄芪甲苷含量测定线性范围为0.12~4.53μg（r=0.998 0）,平均回收率99.88%,RSD为0.44%（n=5）。结论高效液相色谱法专属性、灵敏度、重现性好,可用于该制剂的质量控制。
Objective To establish an HPLC method for the determination of astragaloside in Qianggan Yishen Granule. Methods HPLC was performed on C18 column with methanol-water（75：25） as the mobile phase. The flow rate was 1 mL-min ^-1 and the detector was ELSD. Two points external standard method was used. Results Astragaloside showed a good linearity within the range of 0.12-4.53μg（r=0.998 0）. The average recovery was 99.88%（RSD=0.44%）. Conclusion The method was specific ,accurate and reproducible. It could be used for the quality control of Qianggan Yishen Granule.
出处
《现代中药研究与实践》
CAS
2013年第4期59-60,共2页
Research and Practice on Chinese Medicines
