摘要
目的建立肌苷片溶出度的高效液相色谱(HPLC)测定方法。方法色谱柱为Agilent,clipse XDB-C18(4.6mm×250mm,5μm);流动相:甲醇-水(10:90);流速:1.0mL/min;检测波长为248nm;柱温为25℃。结果肌苷片在2.54~40.60μg/mL的浓度范围内呈良好线性关系(r=0.999 8),平均回收率为100.1%(n=9),RSD为0.3%。结论该方法准确、可靠,与标准方法比较,结果较为满意,可用于肌苷片的溶出度测定。
Objective To establish an HPLC method for dissolution of inosine tablets. Methods Agilent C18 column(4.6 mm×250mm, 5μm)was used with a mobile phase composed of methanol-water(10: 90) )at the flow rate of 1.0 mL,min-l.The detection wavelength was 248 nm and the temperature of the column was 25 ℃ .Results The calibration curve of inosine was linear in the range of 2.54-40.60μg·L^-1(r=0.999 8) ).The average recovery was 100.1% with an RSD of 0.3% (n=9). Conclusion Compared with the standard method, the results were satisfactory, and the method was accurate and reliable, which could be used for the determination of dissolution of inosine tablets.
出处
《现代中药研究与实践》
CAS
2013年第4期61-63,共3页
Research and Practice on Chinese Medicines