红花白千层茎段离体培养研究

In vitro culture of Melaleuca viridiflora ‘Red’ stem

【目的】对红花白千层进行离体培养，为红花白千层的工厂化育苗提供技术支持。【方法】以红花白千层茎段为外植体，探讨不同激素组合对不定芽诱导、丛生芽增殖、生根诱导的影响。【结果】带茎段的萌发腋芽进行增殖培养，可直接诱导形成不定芽，并可形成丛生芽。在MS培养基中添加6．BA0．1～2．0mg／L＋NAA0．01mg／L和6．BA0．1-1．0mg／L＋IBA0．01mg／L组合，芽增殖系数均呈先升高后降低的趋势；培养基MS＋6．BA0．5mg／L＋IBA0．01mg／L最适宜芽增殖，增殖系数达2．2倍。在1／2MS培养基中添加0．1-0．5mg／L的NAA或IBA后，芽苗生根率明显提高，分别为100．0％和56．0％-90．0％，平均生根数为12．6～14．8和3．1-4．8条，以NAA的生根效果明显优于IBA。适宜的生根培养基为1／2MS＋NAA0．1～0.3mg／L。在培养基中添加300mg／L活性炭均不利于芽增殖和生根诱导。以泥炭为栽培基质，采用常规管理的试管苗移植成活率可达99．0％。【结论】茎段离体培养是红花白千层快速繁殖的有效途径。

[Objective]In vitro culture of Melaleuca viriditlora ＇Red＇ was studied in order to provide technique support on its industrialized seedling production. [Method]Stem segment with one axillary bud was used as explant to investigate the effects of different hormone combinations on adventitious bud induction, cluster bud multiplication and rooting induction. [Result]The axillary bud of stem segment could be induced into adventitious bud and cluster buds in multiplication cuhure medium. The multiplication rate of buds in MS medium adding with different combinations 6-BA 0.1-2.0 mg/L ＋ NAA 0.01 mg/L and 6-BA 0.1-1.0 mg/L ＋IBA 0.01 mg/L showed increasing firstly and then decreasing tenderly. MS＋6- BA 0.5 mg/L＋ IBA 0.01 mg/L was the optimizing culture medium of bud multiplication, and the multiplication rate was 2.2 times. Adding 0.1-0.5 mg/L NAA or IBA in 1/2MS medium, the rooting rate of buds increased significantly, which reached 100.0% or 56.0%-90.0%, and the average root number was 12.6-14.8 and 3.1-4.8, respectively. The suitable rooting medium was 1/2MS＋NAA 0.1-0.3 mg/L. AC 300 mg/L was not suitable for bud multiplication and rooting induction. The survival rate of transplanting test-tube plantlets reached 99.0% using peat as culture medium in conventional management. [Conclusion]The regeneration system of Melaleuca viriditlora ＇Red＇ was established, and stem in vitro culture was an effective path for rapid propagation.