类人胶原蛋白-钙螯合物的制备及相关研究

Preparation and Properties Study on the Complex of Human-like Collagen with Calcium

为了制备安全性高且补钙性能优良的补钙制剂,以水体系合成法制备类人胶原蛋白(human-like collagen,HLC)-钙螯合物,探究体系pH值和原料配位比对钙螯合率的影响,并采用紫外-可见吸收光谱和傅立叶红外光谱对制备的类人胶原蛋白-钙螯合物进行分析.确定的最佳工艺条件为:体系pH值5.0,且HLC与CaCl2的质量比10∶1.光谱结果显示:类人胶原蛋白分子和Ca2+之间存在相互作用,且制备的钙螯合物是一种异于HLC的新化合物;羰基和亚氨基参与HLC分子与Ca2+间的螯合反应,且钙螯合物保持类人胶原蛋白分子原有的α-螺旋结构.研究结果为类人胶原蛋白-钙螯合物的大规模制备和进一步应用开发提供了理论依据和研究基础.

The complex of human-like collagen （HLC） with calcium was prepared in aqueous solution and analyzed by UV-vis spectroscopy and FTIR in this research. The effects of pH value and reactant ratio on the coordination efficiency were studied, and the optimum conditions were pH 5.0 and the mass ratio of HLC to calcium chloride 10：1. In addition, it can be concluded from UV-vis spectra that there is interaction between HLC and calcium, and the complex is a new chemical compound different from pure protein. In the complex of Ca,human-like collagen acts as ligand,linking the calcium ions via both groups of C=O and N-H, and the resultant complex retains the helical structure of HLC. The results obtained provide a useful guideline for the large-scale preparation and further research of the HLC-Ca complex.