生物碱参与阿魏种子休眠的调控

Regulation of Alkaloid in Ferula Seed Dormancy

为揭示濒危植物阜康阿魏(Ferula fukanensis Shen.)种子休眠的成因,对阜康阿魏种子分别采用流水漂洗处理后的发芽试验、蒸馏水和NaHCO3溶液浸提液以及化学法提取生物碱中的小麦种子萌发实验,并观察蒸馏水和NaHCO3浸泡阿魏种子对介质pH的影响等,综合分析阿魏种子休眠的成因。流水浸洗处理5 d和20 d的发芽率分别为77%和85%以上,较对照(47%)有显著提高(P<0.01);NaHCO3浸提液对小麦种子萌发的抑制作用显著高于蒸馏水浸提液;用pH较高的NaHCO3溶液和蒸馏水浸泡阿魏种子,该介质的pH向8.0～8.3收敛;化学法提取生物碱对小麦发芽有显著抑制作用,当10 g.(100mL)-1时抑制率达80%。实验结果显示,除脱落酸(ABA)外,还有生物碱在阿魏种子休眠的调控中起重要作用。

Some germination inhibiting substances in the endangered herb Ferula fukanensis seeds were studied so as to find the factors affecting seed dormancy based on the following methods： ① Water treatment with germination test;② Bioassay of water and NaHCO3 leaching liquor;③ Observation of the effect of Ferula fukanensis seeds on the pH values of the extracting media of distilled water and NaHCO3.The results show that the germination percentages of seeds immersed in water for 5 and 20 days were as high as 77% and 85% respectively,and they were significantly higher than 47% of the contrast seeds（P0.01）.The inhibitory effect of NaHCO3 extract on the germination of wheat seeds was significantly higher than that of H2O（P0.05）.The pH values of the distilled water and NaHCO3 extracts converged in a range of 8.0~8.3 after the Ferula seeds were immersed.The inhibitory effect of alkaloid extract on the germination of wheat seeds was significant,and the inhibition rate could be as high as 80% at a concentration level of 10 g·（100L）-1.It could be concluded that the effect of alkaloid on the regulation of seed dormancy of Ferula fukanensis was very important except abscisic acid.