摘要
目的建立人体血清中5-FU含量的测定方法。方法高效液相色谱法测定人血清中5-FU的血药浓度,色谱条件:C18(250 mm×4.6 mm,5μm),流动相:甲醇:水(15:85),检测波长:265 nm,流速:0.8 ml/min,进样量:20μl。结果 5-氟尿嘧啶在1～20μg/ml的线性关系良好(r=0.999 3),在1、5、20μg/ml的日内精密度分别为RSD=5.63%、RSD=2.14%、RSD=2.32%。在1、5、20μg/ml的回收率分别为76.76%、107.87%、98.96%。结论该方法适合应用于人血清中5-FU血药浓度测定。
Objective To establish the method for determination the concentration of 5 -FU in human serum. Methods The concentration of 5 -FU in human serum was determinated by HPLC method, chromatographic condition ： C 18 chromatographic column （ 250 mm ×4.6 mm,5 um） ; moving phase methanol ： water （ 15 ： 85 ） ; detection wavelength： 265nm; flow rate： 0. 8ml/min; sample size： 20 ul. Results 5 -FU in 1 -20 ug/ml has a good linear relationship（ r = 0. 9993 ）. In 1, 5, 20 ug/ml, the RSD of intra - day were 5.63 % ; 2. 14% and 2. 32% respectively. The average recoveries were 76. 76% , 107. 87% and 98.96% respectively. Conclusion This method is suitable for determination of the concentration of 5 - Fu in human serum.
出处
《现代医院》
2013年第9期65-66,共2页
Modern Hospitals
基金
广东省科技计划项目(编号:2011B03170005)
