烤烟根际和非根际土壤微生物典型相关分析

Canonical Correlation Analysis between Tobacco Rhizosphere and Nonrhizosphere Soil Microorganisms

为揭示烟田土壤微生物相互关系,用典型相关分析(CCA)方法研究了烤烟全生育期根际和非根际土壤中微生物之间的相关关系。结果表明:1)在根际土壤中,细菌和氨化细菌数量呈正相关关系;在非根际土壤中,细菌数量同氨化细菌、亚硝化细菌和好气性纤维素分解菌呈正相关关系。2)根际土壤细菌、放线菌数量与非根际土壤细菌数量呈正相关,但是真菌数量在根际和非根际土壤之间相关性不强。3)根际土壤中好气性纤维素分解菌、氨化细菌数量与非根际土壤呈明显的正相关,而好气性固氮菌、亚硝化细菌、硝化细菌和钾细菌数量在根际与非根际土壤间的相关性不强。在植烟土壤3大菌和6类生理功能菌群之间、烟株根际和非根际土壤微生物之间均存在相关关系。

Canonical correlation analysis （CCA） was applied to assess the relationship of rhizosphere and non-rhizosphere microorganisms of flue-cured tobacco plants. The results indicated that （1） the population of bacteria was positively correlated with those of ammonifying bacteria in rhizosphere soil. On the other hand, in non-rhizosphere soil, in addition of ammonifying bacteria, the population of bacteria was also positively correlated with nitrite bacteria and aerobic cellulose decomposing bacteria. （2） The populations of bacterial and actinomycete in tobacco plant rhizosphere could be predicted by the populations of bacterial in non-rhizosphere soil, whereas the population of fungi in rhizosphere was not closely linked to those in non-rhizosphere. （3） The populations of ammonifying and aerobic cellulose decomposing bacteria in rhizosphere were significantly positive correlated with those in non-rhizosphere. However, the populations of aerobic nitrogen fixing bacteria, nitrite and nitrifying bacteria, and kalium solubilizing bacteria in non- rhizosphere were not in close association with those in rhizosphere. In conclusion, there is a strong relationship between three microbial groups and six microbial physiological groups as well as between tobacco rhizosphere and non-rhizosphere soil microbe. The relationship between microbial flora in tobacco rhizosphere is different from non-rhizosphere soil.