烟叶及提取物中茄尼醇的高效液相色谱标准化测定方法研究

Determining Solanesolin in Tobacco Leaves and Its Extracts Using HPLC Standardized Method

为了更好地理解和应用笔者制定的国家标准《烟叶和烟叶提取物中茄尼醇的测定一高效液相色谱法》，笔者详细说明了该方法的技术要点，筛选出最佳的提取条件和皂化条件。高效液相色谱检测条件为：采用WatersAtlantisT3．C18柱（4．6×150mm，3μm），柱温35℃；以异丙醇．甲醇（体积比为30：70）为流动相，流速为1．0mL／min；采用二极管阵列检测器，检测波长213m。实验结果表明，该方法对茄尼醇的检出限为1mg／kg，在0．1～5．0g／100g线性范围内，茄尼醇含量与峰面积的相关系数达0．9999以上；方法加标回收率达到95％以上；相对标准偏差小于5％（n=6）。该方法快速、简便、具有良好的精密度和准确性。

We elucidate a Chinese standard： Determining solanesolin in tobacco leaves and its extracts using HPLC, so that the users can understand and use this standard more proficiently. The best extraction method and condition of saponification was screen out. The HPLC conditions were as follows： Waters Atlantis T3 C18 column （4.6 mm i.d.×150 mm, 3 μm） was used at 35 ℃ with isopropanol-methanol （30：70, v/v） as the mobile phase with a flow rate of 1.0 mL/min, detector UV-213 nm. The detection limit for tobacco leaves and its extracts was l mg/kg. The linear range was from 0.1 to 5.0 g/100g, and correlation coefficients were greater than 0.9999. The recoveries were greater than 95% with relative standard deviations of less than 5%. The method is rapid, simple and accurate.