摘要
从四川藏区高原威代尔冰葡萄酒发酵过程中分离纯化出了异常毕赤酵母(Pichia anomala)菌株,采用18S rDNA D1/D2序列进行了基因鉴定,并对其耐受性进行了研究。结果表明,该酵母菌分别在28℃、pH=4.0或6.0条件下,生长情况较好,具有一定的抗低温性能。随着乙醇、二氧化硫和蔗糖含量的增加,该酵母菌的生长情况受到一定的抑制,该酵母菌对乙醇、二氧化硫和糖均有一定的耐受性。
Pichia anomala was isolated from the fermentation process of the Sichuan Tibetan plateau Vidal icewine and identified using the 18S rDNA D1/D2, its tolerance was also studied. Results showed that the growth ofP. anomala was better at the temperature 28%, pH value 4.0 or 6.0 than that in other conditions. The P. anomala had a certain resistance to low temperature. Moreover, its growth was inhibited with increasing the concentration of alcohol, SO2 and sucrose, respectively. These results indicated that P, anomala showed a certain tolerance against the alcohol, SO2 and sucrose, re- spectively.
出处
《中国酿造》
CAS
2013年第8期39-42,共4页
China Brewing
基金
四川省科技支撑计划项目(2011SZ0284
2012SZ0117
13KCBZ0066)
2012年西华大学食品生物技术重点实验室开放研究基金资助项目(SZjj2012-005)
2013年西华大学研究生创新基金项目(ycjj201346)
关键词
冰葡萄
异常毕赤酵母
分离鉴定
耐受性
ice grape
Pichia anomala
isolation and identification
tolerance
