应用40条引物进行不同品系小鼠RAPD遗传检测的初步研究

Preliminary study of genetic monitoring of various strains of mouse with RAPD-PCR using 40 single primers

目的 :探讨运用RAPD PCR对小鼠进行遗传检测的可能性 ,筛选在各品系小鼠间具有多态性的引物。方法 :运用40条引物对TA1、BALB/c、KM、NIH、SCID、T739、DBA/2、615、BALB/c nu/nu等9个品系小鼠的DNA进行RAPD PCR扩增 ,以琼脂糖凝胶电泳观察结果。结果 :40条引物中只筛选出2条多态性引物 ,其它38条引物扩增结果无品系间多态性。结论 :RAPD PCR方法可以区分9个品系的小鼠 ,是一种适宜于小鼠的遗传检测方法。

Objective: To assess the possibility of genetic monitoring of various strains of mouse with RAPD PCR using 40 single primers. Methods: The DNA of 9 strains of mouse including TA1, BALB/c, KM, NIH, SCID, T739, DBA/2, 615 and BALB/c nu/nu was amplified with RAPD PCR using 40 single primers. The PCR products were resolved with electrophoresis on agarose gel and stained with ethidium bromide. Results: 2 of the 40 single primers resulted in high polymorphism and all the other primers produced monomorphic amplification products in 9 mouse strains. Conclusion: RAPD PCR is an effective technique to proceed genetic monitoring on mice since it is able to distinguish the genetic properties of 9 strains of mouse.