创伤-失血性休克对机体免疫功能的影响

Effects of traumatic-hemorrhagic shock on immune function in rats

目的 :探讨创伤 失血性休克对机体免疫功能的影响。方法 :以大鼠闭合性骨折合并失血性休克为模型 ,于创伤 失血性休克及其复苏后2h、1、2、3、7d处死动物 ,分离并纯化脾淋巴细胞和腹腔巨噬细胞 ,检测脾淋巴细胞增殖能力、IL 2释放、IL 2Rα表达和腹腔巨噬细胞的吞噬能力、MHCⅡ抗原表达及TNF α的分泌。结果 :①创伤 失血性休克后脾淋巴细胞增殖能力降低 ,IL 2释放和IL 2R表达减少 ;②腹腔巨噬细胞的吞噬功能减弱 ,MHCⅡ抗原表达和TNF α释放减少。上述变化在伤后7d尚未恢复到正常水平。结论 :创伤 失血性休克后机体免疫功能受到广泛影响 ,淋巴细胞和巨噬细胞的功能受损 ,并持续较长的时间。

Objective: To investigate the effects of traumatic hemorrhagic shock on immune function. Methods: Splenocytes and peritoneal macrophages were harvested at the 2nd h and on the 1st, 2nd, 3rd and 7th d after the model of closed bone fracture and hemorrhagic shock were established in rats. Then splenocyte proliferation capacity, interleukin 2 release, interleukin 2 receptor expression, peritoneal macrophage phagocytosis, antigen presentation function and TNF α release were determined. Results: All these indexes were decreased after operation as compared with those in the control group. Conclusion: Traumatic hemorrhagic shock induces a marked depression of immune function and the alterations persist for at least 1 week.