荧光全自动血培养仪的临床应用分析

Clinical application of the BACTEC fluorescent instrument

目的 对 2 177份使用仪器的血培养进行统计 ,分析血培养病原菌的分布趋势。同时对两种方法 ,即使用仪器与传统方法的血培养检出率进行比较。方法 应用荧光全自动血培养仪进行培养 ,阳性标本上用全自动微生物分析仪进一步鉴定。同时对未使用仪器前的传统方法也进行统计。结果 2 45份阳性血培养以G-杆菌居首位 (48.2 % ) ,G+ 球菌次之 (47.8% ) ;在儿童血培养中G+ 球菌 6 7% ,G-杆菌 32 % ;使用仪器的检出率为 11.2 5 % ,传统方法为 5 .6 5 %。结论 血培养病原菌以G-杆菌为主 ,G+ 球菌次之 ;而儿童血培养特点是以葡萄球菌属为主 ;使用仪器前后的血培养检出率相差 2倍。

Objectives To determine the distribution of bacteria in blood from patients with fever in our hospital.Methods Blood specimens were cultured into the Bactec Fluorescent Instruments.The colonies of which the specimens yielded growth were identified by the Vitek-Ams.Results In a total of 2177 cultured specimens,245 specimens were obtained positive.of the identified bacteria,48.2% were G - bacilli,47.8% were G + cocci.Conclusions Our study has shown that G - bacilli prevailed over G + cocci in the blood cultures,but the staphylococci were chief bacteria in the children′s blood cultures.