卵泡液中活性氧对体外受精-胚胎移植结局的影响

Influence ofreactive oxygen species in follicular fluid on the outcome of in vitro fertilization and embryo transfer

目的探讨卵泡液中活性氧(ROS)对体外受精-胚胎移植(IVF-ET)结局的影响。方法接受常规IVF受精的夫妇102例,在取卵日收集卵泡液,采用分光光度比色测定法检测过氧化氢(H2O2)、过氧化氢酶(CTA)的含量。根据临床妊娠与否将所得卵泡液分为妊娠组和未妊娠组,分析两组间H2O2、CTA的差异。结果妊娠组卵泡液中的H2O2浓度明显低于未妊娠组(P<0.05),两组患者卵泡液中的CTA浓度无统计学差异。结论高水平ROS可影响卵子的质量及胚胎发育,不利于IVF-ET的结局。

Objective ： To explore the influence of reactive oxygen species in follicular fluid on the outcome of in vitro fertilization and embryo transfer （IVF -ET）. Methods： Follicular fluid from 102 women undergoing IVF was divided into two groups according to whether clinical pregnancy or not： pregnancy group, non - pregnancy group. The concentrations of hydrogen peroxide and catalase were determined with colorimetric. Results： The concentration of hydrogen peroxide in follicular fluid in pregnancy group was significantly lower than the non - pregnant group （P 〈 0. 05）. There was no difference in concentration of catalase between the two groups （ P 〉 O. 05）. Conclusion ： The high concentration of reactive oxygen species could affect the quality of the oocytes and embryo development, and it had no benefit to IVF outcomes.