高盐废水处理的耐盐菌株及其高效除磷特性研究

Efficient Phosphorus Removal from Wastewater by a Newly Isolated Bacterium under High Salinity Condition

筛选出一株能够高效除磷的耐盐菌株HG-1。通过个体形态、菌落特征的观察和16S rRNA基因的序列分析,初步鉴定为盐弧菌属（Salinivibrio sp.）。对菌株HG-1的耐盐性能及其在不同盐度下对磷酸盐的去除效果进行考察,结果表明菌株对盐度的耐受范围为1%~13%,最适盐度为3%。进一步的单因素和正交实验表明,4个环境因素对菌株HG-1磷酸盐去除率影响的强弱为：pH〉碳氮比〉温度〉接种量,最优的除磷条件为pH6.5~7.0,温度30℃,接种量10%,碳氮比9,在该条件下菌株在24小时内对磷酸盐的去除率可达100%。将该菌株应用于高盐废水的处理,可实现磷酸盐的有效去除,具有良好的实际应用价值,为高盐条件下生物除磷难题的解决提供了一条新的途径。

A bacterium capable of phosphorus removal was isolated.Through morphology observation and 16S rRNA gene sequence analysis,the isolate was identified as Salinivibrio sp.（named HG-1）.Salinity tolerance and phosphorus removal efficiency under different salinity conditions of the strain were further investigated.The results showed that HG-1 grew well with the salt content varying from 1% to 13% and achieved the highest phosphorus removal efficiency under salt content of 3%.Furthermore,the single-factor and orthogonal experiment results indicated that the optimal phosphorus removal performance was obtained under the conditions with an initial pH of 6.5–7.0,C/N ratio of 9,temperature of 30°C and inoculation ratio of 10%.Under such a condition,the phosphorus removal efficiency could reach 100% in 24 hours.The strain HG-1 can independently complete phosphorus removal process,and thus could provide a novel and promising alternative for biological phosphorus removal under high salinity conditions.