期刊文献+

不同体细胞评分奶牛血清和乳清中TNF-α、IL-6的检测 被引量:1

The Detection of TNF-α and IL-6 Levels in the Serum and Whey of Cows with Different Somatic Cell Score
下载PDF
导出
摘要 本试验探讨了细胞因子含量的变化与体细胞数的关系,确定其变化是否由乳房炎引起,为今后乳房炎的诊治提供参考依据。依据奶牛生产性能测定体细胞数的检测结果,将82头泌乳奶牛分为低体细胞组(体细胞评分≤2分)、中体细胞组(2分<体细胞评分≤6分)和高体细胞组(体细胞评分>6分),分别检测各组奶牛血清和乳清中细胞因子TNF-α、IL-6的含量,对结果采用SPSS分析。结果显示,随着体细胞数的增加,血清和乳清中TNF-α的含量呈上升趋势;乳清样本各组间TNF-α含量差异显著,高体细胞组显著高于低体细胞组(P<0.05),中体细胞组TNF-α的含量极显著高于低体细胞组(P<0.01)。血清样本中IL-6的含量差异显著,中体细胞组血清中IL-6的含量显著高于低体细胞组(P<0.05),高体细胞组与低体细胞组血清中IL-6的含量差异接近显著(P值为0.059)。 To investigate the correlation between the Somatic Cell Count (SCC) and changes of TNF-a and IL-6 content in serum and whey of dairy cows, 82 lactating dairy cows were divided into low SCC group(Somatic Cell Score〈=2), mid-SCC group(2〈Somatic Cell Score〈=6) and high SCC group(Somatic Cell Score〉6) based on Dairy Herd Improvement (DHI) data report, and the concentrations and changes of TNF-a and IL-6 in serum and whey were detected of the three groups. The results showed that the concentrations of TNF-a in the serum and whey increased with the expansion of the SCC. The concentrations of TNF-a had significant differences in the whey among the three groups, the TNF-a of the high SCC group was significant higher than the low SCC group(P〈0.05), and the mid-SCC group was very significant higher than the low SCC group(P〈0.01). There were also significant differences in the concentrations of IL-6 in the serum among the three groups, the IL-6 of the mid-SCC group was significant higher than the low SCC group(P〈0.05), the high SCC group was nearly significant higher than the low SCC group (P=0.059).
出处 《中国奶牛》 2013年第15期35-37,共3页 China Dairy Cattle
基金 武汉市农科院科技攻关项目<DHI检测对奶牛重要经济性状的预警研究>
关键词 体细胞评分 乳房炎 细胞因子 血清 乳清 Somatic Cell Score Mastitis Cytokines Serum Whey
  • 相关文献

参考文献7

  • 1Schmitz S, Pfaffl M W, Meyer H H D, et al. Short -term changes of m RNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-indnced mastitisLlI. l)omestic Animal Endocrinology, 2004, 26:111-126.
  • 2Ahmed M A, The cytokines of bovine mammary gland: Prospects for diagnosis and therapy[J]. Research in Veterinary Science, 2004, 77:211-222.
  • 3Yang Y L, Li J P, Li K Z, et al. Tumor necrosis factor alpha antibody prevents brain damage of rats with acute necrotizing pancreatitis[J], world Journal of Gastroenterology, 21)t)4, 111(19): 2898-2901).
  • 4刘本君,葛佳瑞,戴波涛,高利.不同程度奶牛乳腺炎IL-8、TNF-α的研究[J].黑龙江畜牧兽医,2011(7):106-107. 被引量:7
  • 5吴朝栋.内毒素诱生的细胞因子及其作用研究进展[J].国外医学(免疫学分册),1995,18(1):11-14. 被引量:16
  • 6Dernfhlk J, Persson Waller K. The xMAP technique can be used for detection of the inflammatory cytokines IL-lbeta, IL-6 and TNF-alpha in bovine samples[J]. Vet lmmunol Immunopathol, 2007, 118(1/2) : 40-49.
  • 7Alhlwaimi A M. The bovine mammary glands cytokines at the periparturient period[J]. Sci J King Faisal Univ (Basic and Applied Sciences), 2007, 8(1):121-130.

二级参考文献4

  • 1RIOLLET C, RAINARD P. Cell subpopulations and cytokine ex- pression in cow milk in response to chronic Staphylococcus aureus infection [ J ]. J Dairy Sci,2001,84 ( 5 ) : 1077 - 1084.
  • 2HOEBEN D,BURVENICH C. Role of endotoxin and TNF -alpha in the pathogenesis of experimentally induced coliform mastitis in periparturient cows [ J ]. J Dairy Res,2000,67 (4) :503 - 514.
  • 3SPICER L J. Receptors for insulin - like growth factor - I and tumor necrosis factor - alpha are hormonally regulated in bovine granulosa and thecal cells[ J]. Anim Reprod Sci ,2001,67:45 -58.
  • 4DERNFALK J,PERSSON WALLER K. The xMAP technique can be used for detection of the inflammatory cytokines IL- lbeta, IL- 6 and TNF - alpha in bovine samples [ J ]. Vet Immunol Immuno- pathol, 2007,118 ( 1/2 ) :40 - 49.

共引文献21

同被引文献25

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部