摘要
本试验探讨了细胞因子含量的变化与体细胞数的关系,确定其变化是否由乳房炎引起,为今后乳房炎的诊治提供参考依据。依据奶牛生产性能测定体细胞数的检测结果,将82头泌乳奶牛分为低体细胞组(体细胞评分≤2分)、中体细胞组(2分<体细胞评分≤6分)和高体细胞组(体细胞评分>6分),分别检测各组奶牛血清和乳清中细胞因子TNF-α、IL-6的含量,对结果采用SPSS分析。结果显示,随着体细胞数的增加,血清和乳清中TNF-α的含量呈上升趋势;乳清样本各组间TNF-α含量差异显著,高体细胞组显著高于低体细胞组(P<0.05),中体细胞组TNF-α的含量极显著高于低体细胞组(P<0.01)。血清样本中IL-6的含量差异显著,中体细胞组血清中IL-6的含量显著高于低体细胞组(P<0.05),高体细胞组与低体细胞组血清中IL-6的含量差异接近显著(P值为0.059)。
To investigate the correlation between the Somatic Cell Count (SCC) and changes of TNF-a and IL-6 content in serum and whey of dairy cows, 82 lactating dairy cows were divided into low SCC group(Somatic Cell Score〈=2), mid-SCC group(2〈Somatic Cell Score〈=6) and high SCC group(Somatic Cell Score〉6) based on Dairy Herd Improvement (DHI) data report, and the concentrations and changes of TNF-a and IL-6 in serum and whey were detected of the three groups. The results showed that the concentrations of TNF-a in the serum and whey increased with the expansion of the SCC. The concentrations of TNF-a had significant differences in the whey among the three groups, the TNF-a of the high SCC group was significant higher than the low SCC group(P〈0.05), and the mid-SCC group was very significant higher than the low SCC group(P〈0.01). There were also significant differences in the concentrations of IL-6 in the serum among the three groups, the IL-6 of the mid-SCC group was significant higher than the low SCC group(P〈0.05), the high SCC group was nearly significant higher than the low SCC group (P=0.059).
出处
《中国奶牛》
2013年第15期35-37,共3页
China Dairy Cattle
基金
武汉市农科院科技攻关项目<DHI检测对奶牛重要经济性状的预警研究>