山药中尿囊素最佳提取方法的优选

Optimization of Extraction Method of Allantoin in Yam

目的:优选尿囊素最佳提取方法。方法:以尿囊素含量为评价指标,采用高效液相色谱法测定20%乙醇与甲醇超声提取、20%乙醇与甲醇回流提取及水提法样品中尿囊素的含量。结果:提取的尿囊素含量由高到低顺序为甲醇超声提取2次>20%乙醇超声2次>甲醇超声1次>20%乙醇超声1次>甲醇回流1次>20%乙醇回流1次>水回流1次。结论:尿囊素以甲醇超声提取2次含量最高。

Objective： To optimize allantoin extraction method from Dioscorea opposita Thunb. Methods： Using allantoin content as the indexes, 20% ethanol and methanol ultrasonic extraction, 20% ethanol and methanol reflux extraction and water formulation samples were determined by HPLC. Results： The allantoin content： Methanol ultrasonic extraction 2 times 〉 20% ethanol ultrasonic 2 times 〉 methanol ultrasonic 1 time 〉 20% ethanol ultrasonic 1 time 〉 methanol reflux 1 time 〉 20% ethanol reflux 1 time 〉 water reflux 1 time. Conclusion： Methanol ultrasonic extraction 2 times has the highest allantoic content.