转抗菌肽B基因和bar基因籼稻植株的再生

Regeneration of Transgenic Indica Rice Plants with a Cecropin B Gene and a bar Gene

运用基因枪法将含有 bar基因和 cecropin B基因的质粒 p CB1导入籼稻品种特青的幼胚细胞 ,筛选后得到 3株转基因植株。PCR检测和 Southern杂交结果表明 ,外源基因已整合到水稻基因组中。转化当代植株表现出对 Basta很强的抗性 。

Immature embryos of indica rice variety Teqing were bombarded with the plasmid pCB1 containing a bar gene and a cecropin B gene. Three transgenic plants were regenerated after selection. The results of PCR assay and Southern blot analysis showed that foreign genes had been integrated into the rice genome. Transformed plants showed high resistance to herbicide Basta and Xanthomonas campestris pv. oryzae.