MNU诱发的水稻巨大胚、甜胚乳两个突变体的RFLP鉴定

RFLP Analysis of Two Rice Mutants Derived from MNU Treatment and Their Original Parent

利用均匀分布于水稻 1 2条染色体上的 72个 RFL P标记与 8种限制性内切酶 (Eco R 、Eco R 、H ind 、 Dra 、 Sca 、Xba 、Bam H 和 Bgl )的不同组合 ,对化学诱变剂 MNU诱变产生的巨大胚、甜胚乳两个突变体及其原始品种金南风三者之间进行 RFL P分析。结果表明 :巨大胚突变体与金南风、甜胚乳突变体与金南风以及两突变体之间的多态性分别为 8.3%、5 .6 %和 1 2 .5 %。巨大胚和甜胚乳两突变体基因突变频率分别为 0 .0 2 2 7和 0 .0 0 70。

Using 72 RFLP markers distributed on 12 chromosomes together with 8 restriction enzymes (EcoRⅠ, EcoRⅤ, Hind Ⅲ, DraⅠ, EcoRⅠ,ScaⅠ, BamHⅠ and BglⅡ), the RFLPs among a rice variety, Kinmaze and its giant embryo and sugary endosperm mutants derived from MNU treatment were studied. The results indicated that RFLPs between ge mutant and Kinmaze, su mutant and Kinmaze, and ge and su mutants were 8.3%, 5.6% and 12.5%, respectively. The mutation frequencies in ge and su mutant were 0.0227 and 0.0070 respectively. Based on the number of restriction enzyme detecting RFLPs, most of mutations were attributed to point mutation.