云南茶树DNA提取和RAPD分子标记初探

Tea Plant 〔 Camellia sinensis (L.) O.Kuntze〕from Yunnan

以宜良宝洪茶、莽水大叶原头茶、波上金苔、云抗 10号 4个云南茶树品种为材料 ,对其DNA提取和RAPD分子标记方法进行了研究。结果表明 :所采用的经改进的CTABDNA微量提取法 ,可以得到高质量的茶树DNA ,分子量大于 2 1kb ,可满足RAPD扩增 ;用 18个不同的随机引物对所提取的 4个品种基因组DNA进行了RAPD分子标记分析 ,其中 3个 (占 16 6 7% )引物在茶树品种间可扩增出多态性产物。建立了云南茶树DNA微量、快速提取和RAPD标记的分析程序 。

In this paper, a method of isolating DNA and RAPD was studied by 4 tea cultivars from Yunnan. The results showed that the good quality DNA of tea plant was gained by the isolating method and the length of DNA fragment was more than 21 kb. The isolated DNA could be used for RAPD analyses; RAPD markers with 18 random primers examined 4 tea cultivars from Yunnan, and 3 of 18 primers reveal polymorphisms among tea cultivars. This RAPD analysis procedure in this experiment lays a good foundation for application of RAPD in tea plant genetic research in Yunnan.