中国纯血马血红蛋白多态性的遗传分析

Genetic Analysis of Haemoglobin Polymorphism in China Thoroughbred Horses

采用等电聚焦电泳对 1 75匹中国纯血马幼驹进行了血红蛋白多态性的检测。结果发现该位点共有 3种基因型 ,由 2个等位基因HbBⅡ 和HbBⅠ 控制。基因型BⅡ /BⅡ、BⅠ /BⅡ和BⅠ /BⅠ的频率分别为 0 .674 3 ,0 .2 85 7和 0 .0 4 0 0 ,其中纯合型BⅡ /BⅡ为优势基因型。基因频率HbBⅡ 为 0 .81 71 ,HbBⅠ 为 0 .1 82 9。通过计算得出该位点的个体识别概率为0 .4 62和亲仔关系排除概率为 0 .1 2

Haemoglobin polymorphism of 175 thoroughbred foals in China was examined by agarose gel isoelectric focusing.The results as follows:three phenotypes were found in the haemoglobin locus,which controlled by two alleles.The genotype frequencies in these foals were BⅡ/BⅡ 0 6743,BⅠ/BⅡ0.2587, BⅠ/BⅠ 0.0400 with predominance of homozygous BⅡ/BⅡ. The gene frequencies were 0.8171 and 0.1829 for HbBⅡ and HbBⅠ.The probability of discrimination power(0.462)and probability of exclusion(0.127) were calculated for the horses.ical features of Trichinella isolates$$$$ JIANG Zhi-min 1,YANG Feng 2,LI Wen-he 3,LI Xue-qi 4,ZHANG Yan-he 4,SONG Ming-xin 5 (1.Hei Long jiang Long Fang Veterinary Medicing Co.Ltd,Harbin 150036,China; 2.Harbin Da Sheng Feedstuff Co.Lat.,Harbin 150038;3.Da Tong Livestock Bureau,Da Qing 163000; 4.Animal Quarantine Station of Zhao Dong City,Zhao Dong 151100;5.Northeast Agricultural University,Harbin 150030,China)Key words:Trichinella spiralis;nurse cell;in vitro Abstract:Some biological features of Trichinella from swine and dog in Hei Long Jiang province.China were first studied,the international standard Trachinella isolates.T.spiralis and T.nativa were used as the control.The experimental results indicated that development time of nurse cells around muscle larvae in mice diaphragms for 4 Trichinella isolates was different,the earliest muscle nurse cell development (encapsulation)dates were at 16 days postinfection(d.p.i)for swine isolate,at 18 d.p.i. for T.spiralis,at 20 d.p.i. for dog isolate and 22 d.p.i. for T.nativa.The interval at which 100% of the muscle larvae became encapsulated was not related to the time of appearance of the first enacpsulated larvae,the 100% level of encapsulation occurred at 32 d.p.i. for dog isolate and T.nativa,at 36 d.p.i. for T.spiralis and at 38 d.p.i. for swines isolate.In vitro,swine isolate and T.spiralis were more productive,with an average of total production of (66.00±7.34) and (76.20±7 57) newborn larvae(NBL)released per female worm at 24 hours of cultivation respectively,dog isolate and T.nativa had lower production,with an average of (28 80±4.30) and (22.00±3.22) NBL/female,respectively. On the basis of the results,it is concluded that Trichinella from swine and dog in Hei Long Jiang province China belong to Trichinella spiralis and Trichinella nativa,respectively.48 and 72 hours after treatment. Key words:Pieris rapae L.;insecticide;β-sitosterol;daphnoritin;chamechromone