摘要
(以生育期相同的)30个梁山慈竹体细胞无性系再生植株和实生苗植株的茎杆为材料,对其纤维素含量和纤维形态进行分析。结果表明,无性系再生植株在纤维素含量和纤维形态方面均发生了较大的变异,再生植株无性系NO.126-1、NO.129的纤维素含量优于对照,达到显著水平(F>F0.05);NO.143的纤维素含量是对照植株的1.16倍,达到极显著水平(F>F0.01);NO.132、NO.40-2和NO.66-1的纤维形态明显优于对照,表明离体诱导的体细胞无性系变异可以应用于梁山慈竹遗传改良。
A total of 30 regenerated plants were obtained simultaneously from callus of the same mature seed embryo in Dendrocalamus farinosus. Then, at the same development stage, cellulose contents and fiber morphology of stems from somaelones and control were further analyzed. The results showed that there were significant differences both in cellulose content and fiber morphology among 30 clones. Somaclones NO. 126-1 and NO. 129 were superior in cellulose content compared with the control. Significantly, cellulose content of NO. 143 was 1. 16-fold of control ( F 〉 F0.01 ). On the other hand, somaclones NO. 132, NO. 40-2 and NO. 66-1 had better fiber quality than the control. This study proved the feasibility of somaclonal variation in vitro in genetic improvement of Dendrocalamus farinosus.
出处
《竹子研究汇刊》
北大核心
2013年第2期12-15,共4页
Journal of Bamboo Research
基金
四川省应用基础研究基金资助项目(05JY029-101)
四川主要丛生竹定向培育技术项目资助(10zx1102)
关键词
梁山慈竹
体细胞无性系变异
再生植株
纤维素含量
纤维形态
Dendrocalamus farinosus
Somaclonal variation
regenerated plants
cellulosecontent
fiber morphology