载脂蛋白E多态性与冠心病患者脂代谢的临床研究

Clinical study of the association between apoE polymorphism and lipid metabolism of patients with CHD

目的 :研究载脂蛋白 E( apo E)多态性与冠心病 ( CHD)及其脂代谢之间的关系。方法 :采用等电点聚焦电泳免疫印迹技术测定 91例 CHD及 10 5例正常对照者的 apo E表型 ,并用酶法测定他们的脂质水平。结果 :CHD组和对照组 apo E表型分布及等位基因频率无显著性差异 ( P >0 .0 5 )。 CHD组不同 apo E表型之间血脂水平比较发现 ,apo E基因多态性与胆固醇 ( TC) ( P <0 .0 1)、低密度脂蛋白胆固醇 ( L DL- C) ( P <0 .0 1)、甘油三酯 ( TG)( P <0 .0 5 )有关 ,即 E4等位基因携带者具有较高的 TC和 L DL - C水平 ,而 E2等位基因携带者具有较低的 TC和 L DL - C水平。 E2和 E4等位基因携带者 TG水平均升高。结论 :apo E基因多态性影响 CHD患者的脂代谢 ,并通过影响脂代谢在 CHD中起重要作用 ,但不是

Objective:To study the relationship between apoE polymorphism and blood lipid level.Method:apoE phenotype was determined in 91 patients with CHD and 105 normal controls by isoelectric focusing and immunoblotting.The lipids of these subjects were measured with enzymes methods.Result:There were no differences in the apoE phenotype distribution and apoE allele frequencies(P> 0.05 )between two groups.Comparasion of values of serum lipid with various apoE phenotype among paitents with CHD revealed that there was correlation between apoE polymorphism and TC(P< 0.01 ),LDL C(P< 0.01 )and TG(P< 0.05 )。Patients carrying E4 were associated with increased TC and LDL C,while those with E2 were assiociated with decreased TC and LDL C.Patients carrying E2 and E4 were associated with increased TG.Conclusion:These data suggest that apoE polyphorphism affects lipid metabolism of patients with CHD,and play an important role in CHD,but it is not an independent risk factor of CHD.