结肠癌组织AKIP1、PKA及P65的表达及意义

Expression and significance of AKIP1, PKA and P65 in colon carcinoma

目的探讨结肠癌组织AKIP1、PKA及P65的蛋白表达情况及其临床意义。方法选择手术切除的结肠癌组织标本及同源癌旁组织标本各37例,采用免疫组化SP法对组织进行染色,观察结肠癌组织及癌旁组织中AKIP1、PKA及P65的表达差异及其相关性,同时观察结肠癌组织AKIP1、PKA及P65表达与结肠癌组织恶性程度及病例分期的相关性。结果结肠癌组织AKIP1、PKA表达强度低于癌旁组织,差异有统计学意义(u=77.42,P<0.05;u=38.09,P<0.05),结肠癌组织AKIP1、PKA阳性表达率(67.6%、75.7%)低于癌旁组织(94.3%、91.9%),差异有统计学意义(χ2=15.51,P<0.05;χ2=10.16,P<0.05);结肠癌组织P65表达强度高于癌旁组织(u=22.07,P<0.05),结肠癌组织P65阳性表达率(91.9%)高于癌旁组织(62.2%)(χ2=27.07,P<0.05),结肠癌组织中AKIP1与PKA表达呈正相关(r=3.106,P<0.05),与P65表达呈负相关(r=-0.40226,P=0.05)。不同TNM分期的结肠癌组织中AKIP1、PKA及P65的表达,两两相比,差异有统计学意义(u=11.92,P<0.05;u=8.17,P<0.05;u=18.02,P<0.05);不同分化程度的结肠癌组织中KIP1、PKA及P65的表达,两两相比,差异有统计学意义(u=22.09,P<0.05;u=12.09,P<0.05;u=27.94,P<0.05)。结论结肠癌组织中存在AKIP1、PKA及P65过度表达现象,并且同结肠癌的生物学行为相关,其可能通过相互调控参与结肠癌的发生发展。

Objective To investigate the protein expression and clinical significance of AKIP1, PKA and P65 in colon carcinoma. Methods 37 tissues of colon carcinoma and tumor-adjacent tissues cut in operation were selected and stained with SP immunohistochemistry, expression difference and correction of AKIP1, PKA and P65 were observed, and correction of AKIPI, PKA and P65 with malignancy and stage were also analyzed. Results Expressive intensity of AKIP1 and PKA were lower in colon carcinoma tissues than those in tumor-adjacent tissues, the differences were sta- tistically significant （u = 77.42, P 〈 0.05; u = 38.09, P 〈 0.05）, and positive rates of AKIP1 and PKA protein were lower in colon carcinoma tissues （67.6%,75.7%） than those in tumor-adjacent tissues （94.3%, 91.9%）, the differences were statistically significant （X2=15.51, P 〈 0.05; X2=10.16, P 〈 0.05）; expressive intensity of P65 was higher in colon carcinoma tissues than those in tumor-adjacent tissues, the difference was statistically significant （u = 22.07, P 〈 0.05）, positive rate of P65 protein was higher in colon carcinoma tissues （91.9%） than that in tumor-adjacent tissues （62.2%）, the difference was statistically significant （~2=27.07, P 〈 0.05）; there was a positive correlation between AKIPI and PKA expression （r = 0.3106, P 〈 0.05）, but a negative correlation between AKIP1 and P65 （r = -0.40236, P 〈 0.05）. There were significant differences of AKIP1, PKA and P65 in different TNM stage （u = 11.92, P 〈 0.05; u = 8.17, P 〈 0.05; u = 18.02, P 〈 0.05） and different differentiation degree of colon cancer tissues （u = 22.09, P 〈 0.05; u = 12.09, P 〈 0.05; u = 27.94, P 〈 0.05）. Conclusion AKIP1 and PKA are low expression and P65 is over ex- pression in colon carcinoma, which related to biological behavior of colon carcinoma, that may participate in occur- rence and progression of colon carcinoma via mutual regulation.