Livin在人成釉细胞瘤中的表达及其意义研究

Expression and significance of Livin in human ameloblastomas

目的检测凋亡抑制蛋白Livin在人成釉细胞瘤(ameloblastomas,ABs)中的表达,探讨ABs发生发展中Livin的作用及其与ABs临床生物学行为的关系。方法从2007—2012年中国医科大学口腔病理科的存档蜡块选取80例ABs标本和10例口腔鳞状细胞癌(OSCC)标本,以及同期口腔颌面外科门诊术中取材的30例ABs标本和智齿拔除术中的10例正常黏膜(NOM),应用免疫组化法、Western-blot方法和RT-PCR法分别检测Livin蛋白和mRNA的表达情况,并进行统计学分析。结果 (1)免疫组化:Livin在NOM中的表达呈阴性;在OSCC中Livin阳性表达;ABs中的阳性率为88.75%(71/80),以中度阳性和强阳性表达为主,定位于牙源性上皮的外周柱状或立方状细胞及中心星网状层胞质中。(2)Western-blot:Livin在ABs中的表达高于NOM(P<0.05),而在ABs和OSCC组织中的表达差异无统计学意义(P>0.05)。(3)RT-PCR:在ABs和OSCC中Livin的表达高于NOM,差异有统计学意义(P<0.05)。结论 ABs中Livin在mRNA水平和蛋白质水平上表达都上调,并且Livin有胞浆的表达,提示Livin在ABs的发生和发展中发挥重要的功能。

Objective To detect the expression of Livin protein in human meloblastomas （ABs）, and to reveal the role of Livin in ABs. Methods By immunohistochemistry , Western-blot and reverse transcription PCR the expressions of liv- in protein were observed on 80 cases of ameloblastomas tissues. Results The positive expression rate of Livin protein was higher in the star of the network and external layer of cells in ameloblastomas. By Western-blot, compared to normal mucosa, the expression of Livin in ABs was higher than that in normal mucosa （P〈0.05） ;we detected the expression of Livin mRNA expression in ABs by reverse transcription PCR, the expression of Livin in ABs was higher than that in nor- mal mucosa （P 〈 0.05） . Conclusion The expression of Livin is associated with AB histogenesis, suggesting that Livin is involved in tumourigenesis.