卡提素生化组分的分离鉴定及质量控制

Studies on identification and quality control of the extracted Bacillus Caimette Guerin (EBCG)

目的 对卡提素中的生化组分进行分离鉴定并建立质量控制方法。方法 采用HPLC ,GC ,TLC ,比色法等对其组分进行分离鉴定 ,以HPGPC测定多糖相对分子质量 ,生物法测定其免疫活性。结果 卡提素中约含 80 %的多糖 ,5 %的核酸、核苷酸以及微量的氨基酸 ;多糖的相对分子质量约为 30万 ;卡提素对小鼠腹腔巨噬细胞吞噬百分率 ( x±s)高达 (5 3.7± 3.3) ,吞噬指数 ( x±s)为 (2 .7± 0 .4) ;玫瑰花结增高率 % (平均值 )为 18.0 %。结论 本品主要成分为多糖、核酸 ,通过测定其含量及活性能达到质量控制目的。

OBJECTIVE To study the isolation and quality control of BECG.METHODS The constituents were isolated and identified by means of HPLC,GC,TCL and colormetry.The polysaccharide molecular weight was determined by HPGPC and the activity was determined by a biological method.RESULTS The EBCG contained about 80% of polysaccartide and 5% of nucleclide and nucleic acid.The polysaccharide molecular weight was 300000.CONCLUSION The composition of EBGC is polysaccharide,nucleotide and microcontent amino acid.