雌激素与内皮细胞凋亡的关系

The Relation Between Estrogen and Apoptosis of Human Umbilical Vein Endothelial Cells (HUVEC)

目的 :观察雌激素 (17β 雌二醇 )对氧化型低密度脂蛋白 (oxLDL)诱导人脐静脉内皮细胞 (HUVEC)凋亡的影响。方法 :以HUVEC为对象 ,观察不同浓度的 17β 雌二醇 (1μmol/L、10 μmol/L和 10 0 μmol/L)对oxLDL(4 μg/L)诱导的凋亡的影响。结果 :不同剂量的 17β 雌二醇对使用oxLDL诱导的HUVEC凋亡减少 70 %～ 93% ,其抑制呈明显的正向量效关系 ,17β 雌二醇 1μmol/L组细胞凋亡占 6 .98%～ 8.6 3 % ,10 0 μmol/L组细胞凋亡占 2 .94%～ 4.85 %。结论 :17β 雌二醇对oxLDL诱导的HUVEC凋亡有明显的抑制作用 ,且在一定浓度范围内呈正向量效关系 ,有利于减少细胞凋亡的发生。

Objective:To observe the effect of estrogen (estradiol-17 beta) on apoptosis of HUVEC induced by oxLDL.Methods:The cultured HUVEC were used to observe the effect of 17 beta-estradiol on its apoptosis induced by oxLDL.The experiment consisted of four groups:the first was control,the second was 1 μmol/L estradiol-17 beta group,the third was 10 μmol/L estradiol-17 beta group and the fourth was 100 μmol/L estradiol-17 beta group.Results:Estradiol-17 beta inhibited the apoptosis of HUVEC induced by oxLDL up to 70%～93%.The inhibition was dosage depended.The ratio of apoptosis was 6.98%～8.63% in group 1 and 2.94%～4.85% in group 4.Conclusion:Estradiol-17 beta inhibits markedly the apoptosis of HUVEC induced by oxLDL.This function is dosage-depended in some concentrations,which contributes to reducing the apoptosis.