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仪器法与菌落计数法检测储粮微生物敏感性的比较 被引量:5

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摘要 目的探讨分析两种方法对储粮中微生物检测的敏感性对比。方法选取从贵州、四川等省粮食储存仓库中收集的水稻、小麦等粮食样品作为研究材料。使用以酶学原理为基础的微生物检测仪与常用检测细菌的平板菌落计数法对上述研究材料进行检测。针对其微生物检测结果的敏感性进行对比分析。结果两种方法对于储粮霉菌孢子的检测敏感性对比差异无统计学意义(P>0.05);而通过对储粮中整体微生物的动态检测,其仪器法敏感性显著优于平板菌落计数法,且结果 (P<0.05)差异具有统计学意义。结论两种检测方法对于静态微生物检测其具有相等的敏感性,而对于生长、繁殖及死亡的动态微生物,其以酶学原理的仪器法检测敏感性更高,且检测结果更加准确,故此方法具有显著的推广价值。
作者 景晓敏
出处 《中国现代药物应用》 2013年第18期252-252,共1页 Chinese Journal of Modern Drug Application
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  • 1[1]Donald W W,Mirocha C J.Chitin as a measure of fungal growth in stored corn and soybean seed[J].Cereal Chem,1977,54(3):466~74.
  • 2[2]Thomas C,Stollman U,Adamed P,et al.Analysis of volatile compounds for detection of molds in stored cereals[J].Cereal Chem,1989,66(4):300~304.
  • 3[3]Thomas B.Volatile metabolites produced by siz fungal species compared with other indicators of fungal growth on cereal grains[J].Applied and Environmental Microbiology,1992,58(8):2599~2605.
  • 4[4]Hassan G,Loye B,Bullerman.Detection of molds in foods and feeds:potential rapid and selective methods[J].Journal of Food Protection,1995,58(12):1389~1395.
  • 5[5]Twiddy D R,Phillips S L.The application in developing countries of immunoassay and rapid chemical methods for detecting postharvest spoilage fungi in srored cereal grains[J].Tropical Science,1995,35:186~199.
  • 6[6]G Kroll R,Frears E R,Bayliss A.An oxygen electrode-based assay of catalase activity as a eapid method for estimating the bacterial content of foods[J].Journal of Applied Bacteriology,1989,66:209~217.
  • 7GB/T4789.2-2008.食品卫生微生物学检验--菌落总数测定[S].1-5.
  • 8SN/T1538.1-2005.培养基制备指南第一部分:实验室培养基制备质量保证通则[S].
  • 9张淑华 沈文堪.TTC试剂抑菌效果试验观察.中国公共卫生学报,1998,(1).
  • 10中华人民共和国国家计量技术规范.测量不确定评定与表示.国家质量技术监督局,1999.

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  • 1邹小波,赵杰文.电子鼻快速检测谷物霉变的研究[J].农业工程学报,2004,20(4):121-124. 被引量:50
  • 2潘天红,陈山,赵德安.电子鼻技术在谷物霉变识别中的应用[J].仪表技术与传感器,2005(3):51-52. 被引量:49
  • 3应永飞,朱聪英,韦敏珏,陈慧华,屈健,陆春波,林仙军,罗成江.液相色谱-串联质谱法测定饲料中14种霉菌毒素及其类似物[J].分析化学,2010,38(12):1759-1764. 被引量:43
  • 4李玉榕,项国波.一种基于马氏距离的线性判别分析分类算法[J].计算机仿真,2006,23(8):86-88. 被引量:46
  • 5Jayas D S. Storing grains for food security and sustainability [J]. Agricultural Research, 2012, 1 ( 1 ) : 21 - 24.
  • 6Khare R, Bedi P S. Aflatoxins: occurrence and their effects -A review[ J]. Current Trends in Biotechnology and Chemi- cal Research, 2012, 2( 1 ) : 15 -25.
  • 7Zhang D H, Li P W, Zhang Q, et al. Production of ultrasen- sitive generic monoclonal antibodies against major aflatoxins using a modified two - step screening procedure [ J ]. Analyti- ca Chimica Aeta, 2009, 636 ( 1 ) : 63 - 69.
  • 8Lai X W, Zhang H, Liu R C, et al. Potential for aflatoxin Bl and B2 production by aspergillus flavus strains isolated from rice samples [ J ]. Saudi Journal of Biological Sciences, 2015, 22(2) : 176 - 180.
  • 9Balasubramanian S, Panigrahi S, Kottapallie B, et al. Eval- uation of an artificial olfactory system for grain quality dis-crimination [ J ]. LWT- Food Science and Technology, 2007, 40(10) : 1815 - 1825.
  • 10Abramson D, Hulasare R, York R K, et al. Mycotoxins, ergosterol, and odor volatiles in durum wheat during granary storage at 16% and 20% moisture content[J]. Journal of Stored Products Research, 2005, 41 ( 1 ) : 67 -76.

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