小剂量顺铂增加放疗诱导Hela细胞凋亡的研究

将 Hela细胞分为空白对照组、单纯放疗组、单纯化疗组和放疗加化疗组四个组 ,采用 DNA片段原位末端监测 TU NEL 法分别检测细胞凋亡率。结果显示单纯放疗、单纯化疗都可诱导 Hela细胞凋亡 ,顺铂可以明显增加放疗诱导的细胞凋亡。表明小剂量顺铂可明显增加放疗诱导的 Hela细胞凋亡 ,为临床放疗时应用小剂量顺铂增敏提供了理论依据。

The Hela cells were divided into four groups(control group,radiation only,chemotherapy only, radiation and chemotherapy) ,a modified in situ TUNEL technique was used to detect the ratio of Hela cell apoptosis. The results showed that each of Radiation and chemotherapy could induce Hela cell apoptosis,sisplatin (chemotherapy) could obviously enhance more Hela cell apoptosis induced by radiation.The study demonstrates that low dose sisplatin can obviously enhance Hela cell apoptosis induced by radiation.The study provides the academic proof for clinical use of low dose sisplatin enhancing Hela cell apoptosis induced by radiation.