胶束电动毛细管色谱法测定水飞蓟素及其制剂中主要有效成分含量

SEPARATION AND DETERMINATION OF EFFECTIVE COMPONENTS IN SILYMARIN AND PREPARATIONS OF SILYMARIN BY MECC

目的 建立一种胶束电动毛细管色谱分离测定水飞蓟素及其制剂Legalon胶囊、益肝灵片中水飞蓟宾、异水飞蓟宾、水飞蓟亭、水飞蓟宁含量的方法。方法 选用缓冲液为 30mmol·L-1硼砂 - 5 0mmol·L-1去氧牛磺胆酸- 2 0mmol·L-1β 环糊精 ,pH为 9 2 ,运行电压 2 0kV ,压差进样 ,进样时间 3s,检测波长为 2 88nm ,温度为室温。内标为芦丁。结果 样品中各组分的平均回收率分别为水飞蓟亭 99 9% ,水飞蓟宁 98 3 % ,水飞蓟宾 99 0 % ,异水飞蓟宾 98 2 %。结论 此法准确、灵敏 ,且能分离水飞蓟宾、异水飞蓟宾的非对映异构体。

AIM To develop a micellar electrokinetic capillary chromatographic (MECC) method for the separation and determination of silybin, isosilybin, silydianin and silycristin in Legalon capsules, Yiganling tablets and silymarin. METHODS A buffer solution containing 30 mmol·L -1 disodium tetraborate, 50 mmol·L -1 taurodeoxycholic acid sodium salt and 20 mmol·L -1 β-cyclodextrin (pH 9 2) was found to be the most suitable electrolyte for this separation. The applied voltage was 20 kV and UV detection wavelength was 288 nm. Rutin was used as internal standard. RESULTS The average recoveries of 99 9% for silycristin, 98 3% for silydianin, 99 0% for silybin and 98 2% for isosilybin were obtained. CONCLUSION The method is sensitive and accurate for the analysis of diastereoisomers of silybin and isosilybin.