金关片镇痛抗炎作用的实验研究

Experimental Study on theAnalgesic andAnti-inflammatory Effects of Jinguan Tablets

目的:观察金关片的镇痛抗炎作用。方法:采用大鼠辐射热刺激实验、小鼠热板法实验、小鼠醋酸扭体实验来观察金关片的镇痛作用,采用小鼠毛细血管通透性实验、小鼠白细胞游走实验、角叉菜胶致小鼠足肿胀实验、小鼠棉球肉芽肿实验、大鼠巴豆性气囊肿实验、大鼠胸膜炎实验观察金关片的抗炎作用,采用小鼠肾上腺质量和维生素C含量测定金关片对肾上腺皮脂功能的影响。结果:金关片高、低剂量组均能提高大鼠辐射热刺激痛阈值,提高小鼠热板法痛阈值,抑制醋酸致小鼠扭体反应,抑制小鼠腹腔毛细血管通透性增高,抑制小鼠白细胞游走,减轻角叉菜胶致小鼠足肿胀的程度,抑制小鼠棉球肉芽肿的形成,抑制大鼠巴豆性气囊肿的形成,减少大鼠胸膜腔中白细胞的数量。金关片组大鼠肾上腺内维生素C含量明显低于空白组(P<0.05或P<0.01)。结论:金关片具有良好的镇痛抗炎作用,且与增强肾上腺皮质功能有关。

Objective：To study the analgesic and anti-inflammatory effects ofjinguan tablets in mice and SD rats.Methods：The analgesic effect ofjinguan tablets was observed by rat radiant heat stimulation experiment,mice hot plate test and mice acetic acid writhing Test.Its anti-inflammatory effect was investigated by mice capillary permeability experiment,mice leukocyte migration experiment,carrageenan-induced mice paw swelling experiment,mice granuloma experiment,rats croton gas cysts experiment and rat pleurisy experiment.Mice adrenal weight and vitamin C content were measured to observe the effects ofjinguan tablets on adrenal gland sebum.Resuits：Jinguan tablets could significantly improve rat radiant heat stimulation pain threshold,increase mouse hot-plate test pain threshold,inhibit acetic acid-induced writhing,peritoneal capillary permeability and leukocyte migration,mitigate the extent of the carrageenan-induced paw swelling,inhibit the formation of granuloma and the formation of croton gas cysts,and reduce the number of white blood cells in the pleural cavity. The adrenal vitamin C content in jinguan tablets group was significantly lower than the control group （ P 〈 0.05 or P 〈 0.01 ） .Conclusion：Jinguan tablets had obvious analgesic and anti-inflammatory effects,and was related to the enhancement of adrenal cortex.