扶正化瘀方影响肝脏胶原生成的拆方配伍研究

A Study on Fu Zheng Hua Yu Fang and its Ingredients on Production of Collagen in the Liver

将扶正化瘀方拆分为扶正组、化瘀组、虫草组、丹参组及丹参加虫草组。制备各组合药血清,分离培养大鼠肝星状细胞,观察含药血清对培养星状细胞增殖活化、Ⅰ型胶原蛋白及其mRNA表达的影响。复制二甲基亚硝胺大鼠肝纤维化模型,以全方及拆方组治疗,观察模型大鼠肝脏羟脯氨酸(Hyp)含量与胶原沉积的变化。结果各组含药血清均可抑制星状细胞的增殖和活化,其中化瘀药效果最明显;各组含药血清均可抑制星状细胞Ⅰ型胶原mRNA及其蛋白的生成,以扶正组效果最佳。对DMN模型肝纤维化的治疗中,以全方组降低肝组织Hyp含量与胶原沉积最为明显,化瘀药与扶正药配伍后减轻胶原生成作用增强,全方具有较好的综合效果。说明扶正化瘀方有较好的抑制星状细胞增殖与胶原基因表达作用,是该方抗肝纤维化作用的重要机理,其中扶正药重在抑制胶原生成,化瘀药长于抑制星状细胞增殖,而全方具有综合效果。

To explore the effect of Fu Zheng Hua Yu Fang and its ingredient (s ) on collagen production we cultured the astrocytes isolated from the dimethyl nitorsamine -induced rat fibrosis model in serum with the extract of the complete prescription and its ingredient (s) and observed their effect in treating the model rats. The ingredients included those for strengthening the body resistance, those for removing stasis, that of Cordyceps, that of Radix Salviae Miltiorrhizae and that of Radix Salviae Miltiorrhizae plus Cordyceps. The results showed that though inhibition of proliferation and activation of the astrocytes was found in all the groups, the effect of the extract of drugs for removing stasis was the strongest. Similarly, inhibition of production of collagen type I and expression of its mRNA was found in all the groups while the effect of drugs for strengthening the body resistance was the strongest. In treating the model rats, the complete prescription was the strongest in reducing the collagen deposition and hyproxyproline content. Combination of drugs for removing stasis with those for strengthening body resistance could further reduce the formation of collagen. The prescription proved to be a comprehensive one in inhibiting collagen production.