大肠癌组织中NF-κB p65的活化与k-ras基因突变的相关性研究

Relationship between NF-κB p65 activation and k-ras gene mutation in colorectal cancer

目的 探讨大肠癌组织中NF-κB p65的活化与k-ras基因突变的相关性。方法 用免疫组织化学方法检测167例已知k-ras基因状态的大肠癌组织中NF-κB p65的核表达。结果 在167例大肠癌组织中，k-ras基因突变59例，突变率35.3 %；NF-κB p65核表达阳性63例，阳性率37.7 ％。不同性别、年龄、ECOG评分、病理类型、病理分化程度、TNM分期、淋巴结转移与否的病例k-ras基因突变率和NF-κB p65核表达差异均无统计学意义（均P＞0.05）。k-ras基因突变的病例中NF-κB p65核表达率50.8 %（30／59），k-ras基因野生型病例中NF-κB p65核表达率30.6 %（33／108）。两者的表达率差异有统计学意义（P＝0.010）。结论 大肠癌组织中NF-κB p65的活化与k-ras基因突变相关。

Objective To investigate the corresponds between NF-κB p65 activation and k-ras mutations. Methods NF-κB p65 activation was analyzed by immunochemistry in 167 colorectal cancer specimens in which the k-ras mutation status was confirmed. Resluts Among 167 colorectal cancer specimens screened , 59 （35.3 %） had k-ras mutations and 63 （37.7 %） had NF-κB p65 activation. k-ras mutations and NF-κB p65 nuclear expression were no significant difference in different sex, age, ECOG score, pathological types, degrees of pathological differentiation, TNM stage, and metastases on lymph nodes （P 〉 0.05）. The positive nuclear expression rate of NF-κB p65 was 50.8 % （30/59） in specimens with k-ras mutations and 30.6 % （33/108） in specimens with wild type k-ras. There was obviously statistical difference between them （P = 0.010）. Conclusion NF-κB p65 activation in coloretal cancer was associated with k-ras mutations.