小麦D型细胞质雄性不育系与保持系叶绿体DNA的RAPD分析

ctDNA RAPD ANALYSIS ON D\|TYPE CMS LINE OF WHEAT AND ITS MAINTAINER LINE

将外源λDNA导入普通小麦品系 81 45 2 7,选出 1稳定的细胞质雄性不育系 ,简称D型不育系。受体 81 45 2 7可以作该不育系的保持系。供体λDNA、受体81 45 2 7、D型不育系及其与鲁麦 1 4号的杂种一代叶绿体DNA的RAPD分析结果显示 ,D型不育系及杂种一代有 1条与供体相同而在受体中不存在的特异带 ,另外有两条特异带在受体和杂种一代中存在 ,而在不育系中消失 ,这两条带可能与育性有关。RAPD分析证明供体DNA片段可以整合插入到受体叶绿体基因组中 ,改变原有的基因表达和调控 。

A stable cms line named D\|type cms line was obtained after injection of exogenous λ DNA into wheat line 814527. Line 814527 can be its maintainer. In order to detect the differences on molecular level among λ DNA, receptor 814527, sterile line and its F 1 hybrid, ctDNA RAPD analysis was made. The results showed that one specific band existed in the doner of the sterile line and its F 1 hybrid, but not existed in the receptor. Two specific bands existed in the receptor and F 1 hybrid, but disappeared in the sterile line. These bands might be related to male sterility. It also proved that the DNA fragment of the donor could be integrated into the genome of receptor, which might disorder the expressing and controlling of the genes, and then bring about mutations.