脑泰方提取物干预大鼠脑缺血后MMP-9-mRNA及PA-mRNA表达研究

The Expression of MMP-9-mRNA and PA-mRNA in Cerebral Ischemia Treated with NTE

目的研究大鼠脑缺血后益气活血中药脑泰方提取物(黄芪、川芎、地龙、僵蚕)干预对缺血区脑水肿及MMP-9-mRNA、PA-mRNA表达的影响。方法随机将SD大鼠分为假手术组、模型组、脑泰方提取物(NTE)低、中、高剂量组(3,9,27g/kg)。各组大鼠预处理ig给药连续3d,每日1次,MCAO模型制备术后ig给药1d。术后1d取材检测各组脑组织含水量,RT-PCR检测MMP-9-mRNA及PA-mRNA表达。结果实验表明高、中NTE组与模型组比较,脑含水量均有明显降低(P<0.05)。NTE高剂量组脑缺血区MMP-9-mRNA、PA-mRNA表达明显降低(P<0.05)。结论脑泰方提取物干预可减轻脑水肿的发生,可能是通过降低MMP-9-mRNA及PA-mRNA的表达,减少MMP-9、PA的生成,发挥保护急性脑缺血血脑屏障的作用。

Objective To observe the expression of MMP-9-mRNA and PA-mRNA when it＇s intervened by traditional herbal medicines NTE （extract of NaoTaiFang,contain HuangQi,Chuan Xiong,Di Long,Jiang Can） in cerebral ischemia and observe effects of cerebral water content in brain. Methods The rats were randomly allotted 5 groups as following：sham operation group, model group,low dose group of NTE （3 g/kg）,medial dose group of NTE （9g/kg）,large dose group of NTE （27 g/kg）. Three days before operation,the corresponding therapy was treated by intragastric administration once every day,After operation,the rats were treated with previous method. Togive materials 24 hours after operation,the water content of brain was detected. MMP-9-mRNA and PA-mRNA in necrotic region were measured by RT-PCR with marketable kit. Results Compared with Model group,the different dose groups of NTE display a lower water content （P〈0.05）;Big dose group of NTE show lower lever of MMP-9-mRNA and PA-mRNA compared with Model group （P〈0.05）. Conclusion NTE can reduce the expression of MMP-9-mRNA and PA-mRNA in cerebral ischemia,then reduce the MMP-9 and PA to protect the BBB,and alleviate cerebral edema.