升麻总苷抗肿瘤活性及其对肿瘤细胞周期的影响

Anti-tumor Activity of Total Glycoside of Cimicifuga foetida L. and Its Effect on Tumor Cell Cycle

目的研究升麻总苷提取物(Total Glycoside of Cimicifuga foetida,TGC)的体内、外抗肿瘤活性及其对人肿瘤细胞周期分布的影响。方法 MTT法检测TGC对人肿瘤细胞株的增殖抑制活性;体内实验观察TGC对小鼠肝癌H22移植瘤和人肝癌裸小鼠异种移植瘤Bel-7402的抑制作用;荧光染色法和流式细胞仪检测其对肿瘤细胞周期的影响。结果 TGC可明显抑制人肝癌HepG2细胞的增殖,其IC50值为(76.16±2.94)mg·L-1;TGC 0.8 g·kg-1剂量可明显抑制小鼠肝癌H22和人肝癌Bel-7402裸小鼠移植瘤的生长;TGC 50 mg·L-1可明显诱导人肝癌HepG2细胞阻滞于G2/M期,呈现出一定的时-效关系。结论 TGC具有明显的体内、外抗肿瘤作用,其活性可能与阻滞肿瘤细胞周期有关。

Objective To investigate the anti-tumor activity of total glycoside of Cimicifuga foetida L.（TGC） and its mechanism. Methods The growth inhibitory effect of TGC on human hepatoma cell line HepG2 was observed by using assay（MTT）;In vivo anti-tumor activity of TGC were evaluated in transplant hepatoma H22 model and human hepatocellular carcinoma Bel-7402 xenograft nude mice model;Cell cycle distribution was assayed by flow cytometry. Results TGC could markedly inhibit the growth of HepG2 （IC50=76.16 ±2.94 mg·L-1）. Compared with the solvent group,TGC showed a significant inhibitory effect on H22 and Bel-7402 at a dose of 0.8 g·kg-1. Furthermore,TGC could arrest HepG2 at G2/M phase at a concentration of 50 mg·L-1 in a time-dependent manner. Conclusion TGC had significant anti-tumor effects both in vivo and in vitro,which might be associated with the arresting of the tumor cell cycle at G2/M phase.