铂钴合金修饰的乙酰胆碱酯酶生物传感器的研制铂钴合金修饰的乙酰胆碱酯酶生物传感器的研制

Preparation of Acetylcholinesterase Biosensor Modified by Pt-Co Alloy

采用恒电位沉积技术，通过聚碳酸酯模板直接在玻碳电极上制得铂钴合金纳米线阵列，将其固定酶制成乙酰胆碱酯酶（AChE）生物传感器，用于抑制剂有机磷农药辛硫磷的测定。考察了电位、pH值、底物浓度等条件对传感器测定辛硫磷的影响。在优化条件下，检测辛硫磷的线性范围为7．00×10^-4-8．20mmol·L^-1,检测下限为2．35×10^-4mmol·L^-1。该传感器具有操作简单、检测范围宽、灵敏度高等优点，用于蔬菜、水果中有机磷农药的测定，效果良好。

Pt-Co alloy nanowire arrays were produced by constant potential deposition technique through the polycarbonate template directly on a glassy carbon electrode, the acetylcholinesterase （ACHE） biosensor was prepared by immobilizing the AchE directly onto the surface, which is used for the determination of organic phosphorus pesticide phoxim inhibitor. The effect of potential, pH and concentration of substrate was investiga- ted. Under the optimized conditions, the currents decrease linearly with the concentration of phoxim in the range of 7.00 × 10^-7 -8.20 × 10^-3 mol · L^-1 ,the detection limit is 2.35 × 10^-7 mol ·L^-1. This biosensor was successfully used to determine pesticides in vegetable and fruit samples with simple operation, wide detec- tion range and high sensitivity etc.