深圳市龙岗区2011～2012年流感样病例呼吸道病毒病原学分析

Etiology of intestinal tractvirus in influenza-like illness patients in Longgang District of Shenzhen during 2011-2012

目的了解深圳市龙岗区流感样病例病原学现况,为呼吸道传染病的预防控制提供依据。方法 2011～2012年采用实时荧光定量PCR法,检测龙岗区801份流感样病例咽拭子样本的流感病毒、腺病毒、鼻病毒、副流感病毒、呼吸道合胞病毒、冠状病毒和人偏肺病毒等7种病毒共15个亚型。结果流感样病例的病毒总阳性率为52.4%(420/801),其中FLU-A检出率最高(27.8%),其后依次为FLU-B(15.7%)、HRV(3.6%)、ADV(2.2%)、RSV-A(1.0%)、PIV-1(0.6%)、RSV-B和CoV-OC43(0.4%)、CoV-229E和hMPV-A(0.2%)、hMPV-B(0.1%),而PIV-2、PIV-3、CoV-NL63和CoV-HKU1四个亚型未检出。7种常见呼吸道病毒检出率高峰分别在2011年2月(69.9%)和2012年1月(85.0%)。7种常见呼吸道病毒以春夏季多发,春夏季与秋冬季比较差异有统计学意义(P<0.05)。FLU以春夏季多发,春夏季与秋冬季比较差异有统计学意义(P<0.05);HRV以冬季多发,冬季与春夏秋季比较差异有统计学意义(P<0.05);ADV、PIV、RSV、CoV、hMPV全年基本为散发分布。不同年龄组的病毒阳性率差别有统计学意义(P<0.05),其中,其中0～4岁组7种常见呼吸道病毒均有检出,5岁以上组主要以FLU为主。结论加强常见呼吸道病毒监测对呼吸道传染病的防控具有重要意义。

Objective To analyze the vetiology of common intestinal virus in influenza-like illness patietns in Longgang District of Shenzhen. Methods Eight hundred and one clinical swabs were detected by Real time PCR. 15 respiratory virus subtypes were detected simultaneously,including influenza virus A/B,adenovirus,human rhinovirus,parainfluenza virus 1/2/3,respiratory syncytial virus A/B,coronavirus 229E/OC43/NL63/HKU1 and human metapneumovirus. Results Totally 420 positive cases were detected and the positive rate was 52.4%. FLU-A was the dominant agent detected （27.8%） ,followed by FLU-B （ 15.7% ） ,HRV （3.6%） ,ADV （2.2%） ,RSV-A （ 1.0% ）, PIV-1（0.6%）,RSV-B and CoV-OC43（0.4%）,CoV-229E and hMPV-A（0.2%）,hMPV-B（0.1%）. But PIV-2,PIV-3, CoV-NL63 and CoV-HKU1 were not found. The respiratory viral detection rates changed regularly within the two years study period. The peak detection rates occurred in February（69.9%） in 2011 and January（ 85.0% ）in 2012. The respiratory most occurred in spring and summer,showing significant difference compared to other two seasons （P〈0.05）. FLU most occurred in spring and summer （P〈0.05）. HRV most occurred in winter,showing significant difference , compared to other three seasons （P〈0.05）. Basically the other respiratory viruses （ADV,PIV,RSV,CoVand hMPV） spread sporadically in the whole year. Also comparing the date from different ages,there was significant difference （P〈0.05）. Additionally,Seven respiratory viruses were identified in age group of 0-4 years,but above age group of 5 years mainly FLU was detected. Conclusions To strengthen the monitoring of common respiratory viruses is of importantt significance for prevention and control of respiratory infectious diseases.