韶关地区乙型肝炎患者HBV亚基因型的分布

Distribution of HBV subgenotypes in hepatitis B patients in Shaoguan area

目的探讨韶关地区乙型肝炎患者血清乙型肝炎病毒(HBV)基因型的分布情况。方法应用PCR法结合琼脂糖电泳方法对378例乙型肝炎患者进行基因分型和检测,根据PCR产物大小直接判定HBV基因型,应用PCR扩增全S基因序列-DNA测序-生物信息学分析方法确定部分样品的亚基因型,并探讨前S区编码情况。结果在378例乙型肝炎患者中B基因型263例占69.6%,C基因型113例占29.9%,BC混合型2例占0.5%。B基因型中HBeAg阳性患者占66.5%,抗-HBe阳性占33.5%。结论广东韶关地区乙型肝炎患者HBV基因型以B型为主,C型次之,少量BC混合型,HBV复制强弱与病毒基因型无关。

Objective To investigate the distribution of HBV genotypes in patients with hepatitis B in ShaoGuan area. MethOds HBV geaotyping and detection in 378 hepatitis B patients were conducted by PCR amplification of S gene sequence of DNA in combination with agarose gel electrophoresis,HBV genotypes were directly determined based on PCR product size and the subgenotypes were identified by DNA sequencing and bioinformaties analysis. Results There were 263 cases of B genotypes,occupied 69.6% of the 378 hepatitis B cases;113 eases of Cgenotype accounted for 29.9% and 2 of BC mixed genotypes accounted for 0.5%. The proportion of B genotype accounted for 66.5% of patients positive for HBeAg and that positive for anti-HBe accounted for 33.5%. Conclusions B subgenotype of HBV patients was predominant in Shaoguan city,followed by C genotype and 8C hybrid,suggesting that the replication intensity of HBV is not associated with the genotype of HBV. Key words： Hepatitis B virus（HBV）, Subgenotype, Distribution features