摘要
目的 探讨平滑肌肌动蛋白 /角蛋白免疫组化双标记法在乳腺疾病诊断以及鉴别诊断中的应用。方法 对乳腺正常组织、良性病变、原位癌、乳腺癌早期浸润以及浸润性癌共 2 0例进行标记。结果 乳腺正常组织、良性病变和叶状囊肉瘤中腺上皮细胞呈鲜红色 ,肌上皮细胞连续性围绕并形成棕色花冠样 ;原位癌癌巢周围肌上皮细胞数目减少 ,呈细绳样连续或不连续围绕 ;乳腺癌早期浸润中早期浸润灶及浸润性癌癌巢周围肌上皮细胞大部分消失或无肌上皮细胞围绕。结论 从乳腺正常组织、良性病变到原位癌、早期浸润和浸润性癌 ,肌上皮细胞的消失是一逐渐发展的过程 。
Objective To explore the significance of double immunolabeling ( DIL) of smooth-muscle actin (SMA) and cytokeratin (CK) in the diagnosis of breast diseases. Methods DIL technique was used to study co-expression of SMA and CK in 20 cases of normal tissues, benign lesions, carcinoma in situ, early invasive carcinomas and invasive carcinomas of the breast. Results After the double staining, epithelial cells were vivid red in color, and myoepithelial cells showed brown, which were continuously around the red cells, producing an appearance of brown 'corolla', in normal tissues and benign cystsarcoma phyllodes of the breast. Myoepithelial layer was mostly or totally absent in early- invasive and invasive carcinomas of the breast. Conclusion Disappearance of myoepithelial cells represents progressive transformation of normal and benign lesions to carcinoma in situ, early-invasive carcinoma and invasive carcinoma of the breast. SMA/CK double immunolabeling is a useful technique in the diagnosis of breast diseases.
出处
《诊断病理学杂志》
CSCD
2000年第3期189-190,I051,共3页
Chinese Journal of Diagnostic Pathology
关键词
乳腺疾病
免疫组化双标记
SMA/CK
诊断
Breast diseases Double immunolabeling Smooth muscle actin Cytokeratin