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乳腺疾病的SMA/CK免疫组化双标记法 被引量:2

Double immunolabeling of smooth-muscle actin and cytokeratin in breast diseases
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摘要 目的 探讨平滑肌肌动蛋白 /角蛋白免疫组化双标记法在乳腺疾病诊断以及鉴别诊断中的应用。方法 对乳腺正常组织、良性病变、原位癌、乳腺癌早期浸润以及浸润性癌共 2 0例进行标记。结果 乳腺正常组织、良性病变和叶状囊肉瘤中腺上皮细胞呈鲜红色 ,肌上皮细胞连续性围绕并形成棕色花冠样 ;原位癌癌巢周围肌上皮细胞数目减少 ,呈细绳样连续或不连续围绕 ;乳腺癌早期浸润中早期浸润灶及浸润性癌癌巢周围肌上皮细胞大部分消失或无肌上皮细胞围绕。结论 从乳腺正常组织、良性病变到原位癌、早期浸润和浸润性癌 ,肌上皮细胞的消失是一逐渐发展的过程 。 Objective To explore the significance of double immunolabeling ( DIL) of smooth-muscle actin (SMA) and cytokeratin (CK) in the diagnosis of breast diseases. Methods DIL technique was used to study co-expression of SMA and CK in 20 cases of normal tissues, benign lesions, carcinoma in situ, early invasive carcinomas and invasive carcinomas of the breast. Results After the double staining, epithelial cells were vivid red in color, and myoepithelial cells showed brown, which were continuously around the red cells, producing an appearance of brown 'corolla', in normal tissues and benign cystsarcoma phyllodes of the breast. Myoepithelial layer was mostly or totally absent in early- invasive and invasive carcinomas of the breast. Conclusion Disappearance of myoepithelial cells represents progressive transformation of normal and benign lesions to carcinoma in situ, early-invasive carcinoma and invasive carcinoma of the breast. SMA/CK double immunolabeling is a useful technique in the diagnosis of breast diseases.
出处 《诊断病理学杂志》 CSCD 2000年第3期189-190,I051,共3页 Chinese Journal of Diagnostic Pathology
关键词 乳腺疾病 免疫组化双标记 SMA/CK 诊断 Breast diseases Double immunolabeling Smooth muscle actin Cytokeratin
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  • 1[1]Raymond WA, Laeong AS. Asessment of invasiom in breast lesions usingantibodies to basement membrane components and myoepithelial cells.Pathology, 1991,23: 291-297
  • 2[2]Prasad Ml, Osborne MP, Hoda SA, et al. Observations on the histopathologic diagnosis of microinvasive carcinoma of the breast. Anat Pathol,1998,3:209 - 232
  • 3[3]Damiani S,Ludvikova M, Tomasic G, et al. Myoepithelial cells and basallamina in poorly diffentiated in situ duct carcinoma of the breast. An immunocytochemical study. Virchows Arch, 1999,434:227-234
  • 4[4]Prasad ML, Hyjek E,Giri DD,et al. Double inmmunolabeling with cytokeratin and smoothmuscle actin in confirming early invasive carcinoma ofbreast. Am J Surg Pahtol, 1999,23:176-181

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