摘要
目的建立测定野鸦椿药材中鞣花的含量高效液相色谱法。方法色谱柱为WaterssymmetryC18柱（150mm×4．6mm，5μm），流动相为甲醇-0．1％磷酸3z（36：64），流速1mL／min，柱温为40℃，检测波长为254nm。结果鞣花酸进样量在0．129～2．06Ixg范围内与峰面积呈良好线性关系（r=0．9994），平均加样回收率为100．57％，RSD为2．43％（n=6）。结论该方法线性关系、精密度和准确度良好，专属性强，可作为野鸦椿药材中鞣花酸的质量控制方法。
Objective To establish a HPLC method for the determination of the ellagic acid content in Euscaphis Japonica（ThuMb. ）. Methods ＇File chromatographic separation was performed on the Waters symmetry C]s column （150 mm×4.6 mm, 5 μm） with methanol-0. 1% H3PO4 solution（36：64） as the mobile phase, the flow rate was 1 mL/min and the column temperature was 40 ℃. The detection wave length was 245 nm. Results The linearity of ellagic acid was in the range of 0. 129- 2.06 μg（ r =0. 999 4）, the average recovery rate was 100. 57%, RSD was 2.43% （n =6）. Conclusion This method has good linearity, precision and accuracy strong speci- ficity. It can be used for the quality control method of ellagic acid in Euscaphis Japonica（ThuMb. ）.
出处
《中国药业》
CAS
2013年第17期16-18,共3页
China Pharmaceuticals
基金
贵州省中药现代化科技产业研究开发专项资金项目
项目编号:黔科合中药专字[2007]5014号
关键词
高效液相色谱法
野鸦椿
鞣花酸
high performance liguid chromatography
Euscaphis Japonica（ThuMb. ）
ellagic acid
