纳米载体PEI介导IGF1R-siRNA对A549肺癌细胞增殖活性影响的初步研究

Preliminary study on nanometer carrier PEI mediated IGF1R-siRNA to supress human lung cancer A549 cells in vitro

目的 以纳米载体聚乙烯亚胺（polyethylenimine,PEI）介导人胰岛素样生长因子1受体（insulin-like growth factor 1 receptor,IGF1R）小干扰（small interfering RNA,siRNA）质粒转染A549肺癌细胞,并初步观察沉默IGF1R基因对A549肺癌细胞体外增殖活性的影响.方法 设计IGF1R小干扰序列,构建PSUPER-IGF1R-siRNA质粒,PEI介导转染A549肺癌细胞,台盼蓝染色法和MTT法测定细胞存活率.结果 测序结果显示构建质粒与基因库IGF1R基因序列一致.PEI介导PSUPER-IGF1R-siRNA质粒转染A549肺癌细胞后,台盼蓝染色法和MTT 法检测结果显示细胞存活率降低,与生理盐水组和pSUPER-EGFR质粒对照组间的差异有统计学意义（P〈0.05）.结论 pSUPER-IGF1R-siRNA质粒构建成功,PEI介导其转染A549肺癌细胞后对细胞有生长抑制作用.

Objective To construct the small interfering RNA （siRNA） plasmid of insulin - like growth factor 1 re-ceptor （IGF1R） gene and transfeet human lung cancer A549 cells mediated by nanometer carrier polyethylenimine （PEI） and observe its growth inhibition effect. Methods The siRNA sequence targeting IGF1R was designed and the PSUPER-IGF1R-siRNA plagmid was eonstrucleted and transfected into A549 cells by PEI nanoparticles. The growth inhibition effect was monitored by trypan blue staining and MTF assay. Results Gene sequencing results of constructed plasmids showed consistent with the IGF1R gene sequences in GenBank. The survival rate of A549 cells transfeeted PSUPER - IGF1R - siRNA plagmid was significantly low compared with two contol groups （ P 〈 0.05 ）. Conclusion The PSUPER - IGF1R - siRNA plagmid was successfully constructed. The growth of A549 cells was inhibited by PSUPER - IGFI R - siRNA plagmid transported by nanometer carrier PEI.