姜黄素对阿霉素诱导的大鼠心脏重构及心功能改变的研究(英文)

Effect of curcumin on cardiac remodeling and heart function change induced by adriamycin in rats

目的:研究姜黄素对阿霉素引起的大鼠心脏重构及心功能改变的作用。方法:50只SD大鼠随机分为空白对照组,阿霉素组,姜黄素干预组,观察大鼠的一般情况(体重、毛色、饮食、活动能力),以超声心动图观察大鼠左室射血分数(LVEF)、左室短轴缩短分数(LVFS)、左室舒张末期内径(LVEDd),称量大鼠心脏重量,以免疫组化法观察大鼠心肌Ⅰ、Ⅲ型胶原的变化。结果:实验后,与空白对照组相比,阿霉素组大鼠心脏重量[(168.53±26)mg比(208.34±31)mg]、心脏重量/体重[(0.36±0.06)比(0.52±0.08)]显著增加,体重[(469.27±34)g比(428.47±45)g]显著减轻;LVEDd[(4.87±0.19)mm比(7.49±0.83)mm]明显增加,LVEF[(69.53±6.25)%比(41.46±4.38)%]、LVFS[(45.83±3.79)%比(17.25±2.92)%]明显降低(P均<0.05);Ⅰ、Ⅲ型胶原蛋白表达率明显升高(P<0.01)。与阿霉素组大鼠比较,姜黄素组大鼠体重(452.46±38)g显著增加,心脏重量(178.73±38)mg、心脏重量/体重(0.39±0.07)显著减轻,LVEDd(5.96±0.65)mm显著降低,LVEF(53.12±5.43)%LVFS(36.57±3.66)%显著增加(P均<0.05);Ⅰ、Ⅲ型胶原蛋白表达率明显降低()。结论:姜黄素能改善阿霉素引起的鼠心脏重构和心功能改变。

Objective：To study the effect of curcumin on cardiac remodeling and heart function change induced by adriamycin in rats.Methods：A total of 50 SD rats were randomly divided into blank control group（n=10）,adriamycin group（n=20）and curcumin intervention group（n=20）.General conditions of rats,including weight,hair color,diet and activity capacity,were observed.Left ventricular ejection fraction（LVEF）,left ventricular fractional shortening（LVFS）and left ventricular end-diastolic dimension（LVEDd）were observed by echocardiography.Rat heart was weighed and levels of cardiac collagen typeⅠandⅢ were observed using immunohistochemistry method.Results：Compared with blank control group after experiment,there were significant increase in heart weight[（168.53±26）mg vs.（208.34±31）mg],heart weight/body weight[（0.36±0.06）vs.（0.52±0.08）],and significant decrease in body weight[（469.27±34）g vs.（428.47±45）g];significant increase in LVEDd [（4.87±0.19）mm vs.（7.49±0.83）mm],significant decrease in LVEF[（69.53±6.25）% vs.（41.46± 4.38）%]and LVFS[（45.83±3.79）% vs.（17.25±2.92）%]in adriamycin group,P＆lt;0.05 all,significant increase in expression rates of collagen typeⅠandⅢ,P＆lt;0.01.Compared with adriamycin group,there were significant increase in body weight（452.46±38）g,significant decrease in heart weight（178.73±38）mg,heart weight/ body weight（0.39±0.07）;significant decrease in LVEDd（5.96±0.65）mm and significant increase in LVEF（53.12±5.43）% and LVFS（36.57±3.66）%,P ＆lt;0.05 all;significant decrease in expression rates of collagen typeⅠ andⅢin curcumin group,P ＆lt;0.01.Conclusion：Curcumin can improve cardiac remodeling and heart function for heart impairment induced by adriamycin in rats.