摘要
目的:研究姜黄素对阿霉素引起的大鼠心脏重构及心功能改变的作用。方法:50只SD大鼠随机分为空白对照组,阿霉素组,姜黄素干预组,观察大鼠的一般情况(体重、毛色、饮食、活动能力),以超声心动图观察大鼠左室射血分数(LVEF)、左室短轴缩短分数(LVFS)、左室舒张末期内径(LVEDd),称量大鼠心脏重量,以免疫组化法观察大鼠心肌Ⅰ、Ⅲ型胶原的变化。结果:实验后,与空白对照组相比,阿霉素组大鼠心脏重量[(168.53±26)mg比(208.34±31)mg]、心脏重量/体重[(0.36±0.06)比(0.52±0.08)]显著增加,体重[(469.27±34)g比(428.47±45)g]显著减轻;LVEDd[(4.87±0.19)mm比(7.49±0.83)mm]明显增加,LVEF[(69.53±6.25)%比(41.46±4.38)%]、LVFS[(45.83±3.79)%比(17.25±2.92)%]明显降低(P均<0.05);Ⅰ、Ⅲ型胶原蛋白表达率明显升高(P<0.01)。与阿霉素组大鼠比较,姜黄素组大鼠体重(452.46±38)g显著增加,心脏重量(178.73±38)mg、心脏重量/体重(0.39±0.07)显著减轻,LVEDd(5.96±0.65)mm显著降低,LVEF(53.12±5.43)%LVFS(36.57±3.66)%显著增加(P均<0.05);Ⅰ、Ⅲ型胶原蛋白表达率明显降低()。结论:姜黄素能改善阿霉素引起的鼠心脏重构和心功能改变。
Objective:To study the effect of curcumin on cardiac remodeling and heart function change induced by adriamycin in rats.Methods:A total of 50 SD rats were randomly divided into blank control group(n=10),adriamycin group(n=20)and curcumin intervention group(n=20).General conditions of rats,including weight,hair color,diet and activity capacity,were observed.Left ventricular ejection fraction(LVEF),left ventricular fractional shortening(LVFS)and left ventricular end-diastolic dimension(LVEDd)were observed by echocardiography.Rat heart was weighed and levels of cardiac collagen typeⅠandⅢ were observed using immunohistochemistry method.Results:Compared with blank control group after experiment,there were significant increase in heart weight[(168.53±26)mg vs.(208.34±31)mg],heart weight/body weight[(0.36±0.06)vs.(0.52±0.08)],and significant decrease in body weight[(469.27±34)g vs.(428.47±45)g];significant increase in LVEDd [(4.87±0.19)mm vs.(7.49±0.83)mm],significant decrease in LVEF[(69.53±6.25)% vs.(41.46± 4.38)%]and LVFS[(45.83±3.79)% vs.(17.25±2.92)%]in adriamycin group,P&lt;0.05 all,significant increase in expression rates of collagen typeⅠandⅢ,P&lt;0.01.Compared with adriamycin group,there were significant increase in body weight(452.46±38)g,significant decrease in heart weight(178.73±38)mg,heart weight/ body weight(0.39±0.07);significant decrease in LVEDd(5.96±0.65)mm and significant increase in LVEF(53.12±5.43)% and LVFS(36.57±3.66)%,P &lt;0.05 all;significant decrease in expression rates of collagen typeⅠ andⅢin curcumin group,P &lt;0.01.Conclusion:Curcumin can improve cardiac remodeling and heart function for heart impairment induced by adriamycin in rats.
出处
《心血管康复医学杂志》
CAS
2013年第4期316-320,F0002,共6页
Chinese Journal of Cardiovascular Rehabilitation Medicine
基金
黑龙江省卫生厅科研课题(2009-322)~~
关键词
I型
胶原III型
心室功能
Curcumin
Ventricular remodeling
Collagen type I
Collagen type III
Ventricular function