β-谷甾醇对原代大鼠海马神经元细胞活力的毒性

The toxicity of β-sitosterol on the vitality of hippocampal neuron cell in original generation rats

目的:研究β-谷甾醇对正常和痫性放电海马神经元的毒性作用。方法:采用24h内新生Wistar大鼠为细胞源,用Neurobasal培养液培养海马神经元9d后,加入无镁离子处理3h,诱导其痫性放电,加入β-谷甾醇(浓度),作用24h,MTT法检测β-谷甾醇对海马神经元的无毒和对原代大鼠海马神经元癫痫样细胞最佳作用时间的影响。结果:成功分离出大鼠原代海马神经元细胞,β-谷甾醇在20μmol/L浓度下对海马神经元细胞无毒性,最佳作用时间为24h。结论:浓度20μmol/L,最佳作用时间为24h,β-谷甾醇对海马神经元没有毒性,可用于癫痫治疗。

Objective： To study sitosterol toxic effects on normal and epileptic discharge hippocampal neurons.Methods： Wistar rats neonatal in 24h were used as cell source,with Neurobasal culture medium cultured for 9 days,without Mg2＋ treated for 3 h,to induce epileptic discharge,and sitosterol alcohol（concentration） was added to react for 24h.MTT assay was used to check the effect of beta-sitosterol on hippocampal neurons non-toxic and primary rat hippocampal neurons in epileptic cells for the best time.Results：We succeeded in isolating the newborn rat hippocampal nerve cells,and the non-toxic concentration of sitosterol was 20μg/mL,and the best time was 24h.Conclusion： Sitosterol concentration of 20μmol/L,for 24 hours,are not toxic for hippocampal neurons,which can be used for the treatment of epilepsy.